Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-20
2000-05-30
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 912, 435290, 435808, 422 50, 422 61, 422 63, 422 681, 422 81, 422 99, 422141, 422145, 422146, 422149, 935 1, 935 16, 935 17, C12Q 168
Patent
active
060689781
ABSTRACT:
Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a one or two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction/detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. Microprocessor control controls the heat applied by the second element independently of the heat applied by the first element. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target. Images are captured and digitized and subjected to statistical analysis to improve the accuracy of the method.
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Bouma Stanley R.
Gordon Julian
Kotlarik John J.
Solomon Natalie A.
Zaun Peter
Abbott Laboratories
Horlick Kenneth R.
Taylor Janell E.
Yasger Paul D.
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