Apparatus and method for the rapid spectral resolution of confoc

Details Apparatus and method for the rapid spectral resolution of confoc Apparatus and method for the rapid spectral resolution of confoc

1999-01-14

2000-10-17

Font, Frank G.

Optics: measuring and testing

By dispersed light spectroscopy

Utilizing a spectrometer

356318, 356346, 359368, 2504581, 2504591, G01J 328, G01J 330

Patent

active

061340022

ABSTRACT:
A confocal scanning microscope apparatus and method is used to rapidly acquire spectrally resolved images. The confocal scanning microscope apparatus includes optics used to simultaneously acquire at least two points along a scan pattern on a sample plane of a sample, wherein the points include regions of the sample represented by at least two pixels. A detection arm is placed in the path of the light reflected, scattered, or emitted from the sample plane comprising a spectrometer having a slit for receiving such light and a detector array placed behind the spectrometer. The image corresponding to the at least two points is recorded on the first axis of said detector and the spectral resolution thereof is simultaneously recorded on the second axis of said detector. The confocal scanning microscope apparatus and method offers significant improvements to the current techniques for genetic sequencing, as well as significant improvements to traditional confocal scanning microscope applications.

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A. Feofanov et al., "A new confocal stigmatic spectrometer for micro-Raman and microfluorescence spectral imaging analysis: Design and applications," American Institute of Physics, vol. 66 (No. 5), p. 3146-3158, 1995.

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