Apparatus and method for the manipulation and testing of...

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory...

Reexamination Certificate

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C422S051000, C422S051000, C436S501000, C436S524000, C436S527000, C436S533000, C435S007210, C435S007500, C435S007600, C435S007900, C536S023100, C356S302000, C356S305000, C356S330000, C356S904000, C356S929000, C356S958000, C251S315040, C251S315040, C251S315040, C251S315040

Reexamination Certificate

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07052650

ABSTRACT:
Apparatus for the manipulating and testing of molecules and in particular of DNA comprising a surface on which the molecule is anchored on multiple points at one end and a paramagnetic bead on which said molecule is anchored on multiple points at its other end, magnetic means for applying a force to the bead, said magnetic means being used to control the stretching and rotation of said bead and molecule, optical magnification means and a camera for the visualisation of said bead, computer means to which the images of the camera are transmitted, said computer means comprising means for analyzing the motions of the bead. A method for the manipulating and testing of molecules and in particular of DNA in which a molecule is anchored at one end to a fixed surface and at its other end to a paramagnetic bead wherein said molecule is anchored on multiple points at each of said ends.

REFERENCES:
patent: 3860344 (1975-01-01), Garfunkel
patent: 5486457 (1996-01-01), Butler et al.
patent: 5532132 (1996-07-01), Wang et al.
patent: 5976896 (1999-11-01), Kumar et al.
P. Cluzel, et al., “DNA: An Extensible Molecule”,Science, 271, No. 5250, (1996), pp. 792-794.
S.B. Smith, et al., “Overstretching B-DNA: The Elastic Response of Individual Double-Stranded and Single-Stranded DNA Molecules,”Science, 271, No. 5250, (1996), pp. 795-799.
S.B. Smith, et al., “Direct Mechanical Measurements of the Elasticity of Single DNA Molecules by Using Magnetic Beads,”Science, 258, No. 5085, (1992), pp. 1122-1126.
C. Bustamante, et al., “Entropic Elasticity of Λ-Phage DNA,”Science, 265, No. 5178, (1994), pp. 1599-1600.

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