Apparatus and method for the generation, separation, detection,

Details Apparatus and method for the generation, separation, detection, Apparatus and method for the generation, separation, detection,

1995-05-09

2000-01-25

McKane, Elizabeth

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204606, 204616, 204466, 4352872, 4352873, 356344, G01N 2726, G01N 27447

Patent

active

06017434&

ABSTRACT:
This invention is an integrated instrument for the high-capacity electrophoretic analysis of biopolymer samples. It comprises a specialized high-voltage, electrophoretic module in which the migration lanes are formed between a bottom plate and a plurality of etched grooves in a top plate, the module permitting concurrent separation of 80 or more separate samples. In thermal contact with the bottom plate is a thermal control module incorporating a plurality of Peltier heat transfer devices for the control of temperature and gradients in the electrophoretic medium. Fragments are detected by a transmission imaging spectrograph which simultaneously spatially focuses and spectrally resolves the detection region of all the migration lanes. The spectrograph comprises a transmission dispersion element and a CCD array to detect signals. Signal analysis comprises the steps of noise filtering, comparison in a configuration space with signal prototypes, and selection of the best prototype. Optionally post-processing is done by a Monte-Carlo simulated annealing algorithm to improve results. Optionally, an array of micro-reactors can be integrated into the instrument for the generation of sequencing reaction fragments directly from crude DNA samples.

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