Apparatus and method for testing the sufficiency of sterilizatio

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435294, 435296, 435299, 435300, 435311, 435810, 436 1, 436807, 436810, 206219, 206222, 215227, 215DIG8, C12Q 122, C12M 124, B65D 2508

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045282684

ABSTRACT:
Apparatus and method for testing the sufficiency of sterilization includes a test tube having a distal end in which a bacterial spore element is disposed. A sealed glass ampule containing a sterile liquid culture medium is disposed in the test tube and a plunger having a fenestration closed with a hydrophobic filter is fitted slidingly in the open end of the test tube. In use, the apparatus is exposed to sterilization, and thereafter the plunger is pushed down into the test tube to engage the ampule to slide the latter into engagement with an ampule-engaging means to thereby fracture or break the ampule so that the contents of the ampule are released into the test tube to contact the spore element, whereby the apparatus is then subjected to incubation, the sufficiency of the sterilization being thereby determined.

REFERENCES:
patent: 3440144 (1969-04-01), Anderson
patent: 4304869 (1981-12-01), Dyke

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