Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1997-08-14
2000-05-09
Beisner, William H.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
4352865, 435401, C12M 300, C12N 500
Patent
active
060603062
ABSTRACT:
An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing cartilage constructs is disclosed. The apparatus generally includes a treatment chamber and a means for varying the fluid flow and pressure within the treatment chamber during seeding and culturing. The application of pressure to the cartilage construct improves both the uniformity of seeding throughout the construct and facilitates the flow of nutrients to and removal of waste products from cells embedded in the construct. In this manner, a tissue-engineered cartilage construct with cells and their fibers oriented in a manner which is more likely to possess long term dimensional stability and the patency of cartilage with normal physiologic function may be formed.
REFERENCES:
patent: 3734851 (1973-05-01), Matsumura
patent: 3883393 (1975-05-01), Knazek et al.
patent: 4201845 (1980-05-01), Feder et al.
patent: 4417861 (1983-11-01), Tolbert
patent: 4639422 (1987-01-01), Geimer et al.
patent: 4762794 (1988-08-01), Nees
patent: 4804628 (1989-02-01), Cracauer et al.
patent: 4988623 (1991-01-01), Schwarz et al.
patent: 5026650 (1991-06-01), Schwarz et al.
patent: 5043260 (1991-08-01), Jauregul
patent: 5068195 (1991-11-01), Howell et al.
patent: 5081035 (1992-01-01), Halberstadt et al.
patent: 5153131 (1992-10-01), Wolf et al.
patent: 5153132 (1992-10-01), Goodwin et al.
patent: 5153133 (1992-10-01), Schwarz et al.
patent: 5155034 (1992-10-01), Wolf et al.
patent: 5155035 (1992-10-01), Schwarz et al.
patent: 5230693 (1993-07-01), Williams et al.
patent: 5266480 (1993-11-01), Naughton et al.
patent: 5308764 (1994-05-01), Goodwin et al.
patent: 5376110 (1994-12-01), Tu et al.
patent: 5792603 (1998-08-01), Dunkelman et al.
Eskin et al. `Behavior of Endothelial Cells Cultured . . . ` Artificial Organs. vol. 7, No. 1 (1983), pp. 31-37.
Atikinson et al.; Biochemical Engineering and Biotechnology Handbook; pp. 476-487 (1991).
Halberstadt et al., "The In Vitro Growth of a Three-Dimensional Human Dermal Replacement Using a Single-Pass Perfusion System," Biotechnology and Bioengineering 43:740-746 (1994).
Applegate Mark A.
Flatt James H.
Peterson Alvin
Advanced Tissue Sciences Inc.
Beisner William H.
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