Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Solid support and method of culturing cells on said solid...
Patent
1995-06-07
1998-12-08
Beisner, William H.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Solid support and method of culturing cells on said solid...
4352841, 4352865, 4352891, 623 2, C12N 500
Patent
active
058468285
ABSTRACT:
An apparatus and method for sterilizing, seeding, culturing, storing, shipping, and testing heart valves is disclosed. Specifically, the present invention relates to an apparatus and method for dynamically seeding and culturing heart valves with human cells, resulting in a heart valve populated with viable human cells. In a preferred embodiment, the apparatus includes a fluid reservoir, a pneumatic pressure chamber, a pressure source for providing a varying pressure to the pressure chamber, and a heart valve holder. In an alternative exemplary embodiment, the apparatus includes a fluid reservoir, a pump, a venturi tube, a heart valve chamber, a heart valve holder, and a valve connected to a timer. In each embodiment, the components are combined in a fluid circuit to create a variable cycling and pulsatile fluid flow for seeding and culturing which can closely resemble the physiological conditions found in the human heart.
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Bennett John
Chesla Scott
Gee Jason
Landeen Lee K.
Peterson Alvin
Advanced Tissue Sciences
Beisner William H.
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