Apparatus and method for shear breakage of polynucleotides

Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o

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536 231, 536 254, 4352851, 4352865, 4352866, 422 50, 422 63, 422243, 422281, 422295, 935 76, 935 85, G01N 3300, C12M 304, B01D 1102, C12N 1502

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058468323

ABSTRACT:
Apparatus and method for producing a defined size distribution of polynucleotide fragments having a selected mean size range between about 100 and 20,000 base pairs. The method includes passing a suspension of polynucleotide fragments through an apparatus containing a capillary with an orifice near the outlet end of the capillary.

REFERENCES:
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Reese, H.R., and Zimm, B.H., "Fracture of Polymer Chains in Extensional Flow: Experiments with DNA and a Molecular-Dynamics Simulation," J. Chem. Phys. 92(4): 2650 (1990).
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