Apparatus and method for separating ribonucleic acid

Details Apparatus and method for separating ribonucleic acid Apparatus and method for separating ribonucleic acid

2007-09-25

2007-09-25

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024310, C536S024320

Reexamination Certificate

active

10704707

ABSTRACT:
An RNA separation method includes injecting a reaction solution into a reaction region which contains a covalent linking of specific RNA probe for hybridizing with the reaction solution; adjusting a moderate hybridization temperature in the reaction region; moving the remainder of the reaction solution to a transition region by using a pneumatic actuator; adding a buffer solution to the reaction region, and heating the reaction region to a moderate denature temperature by a heating apparatus; moving a waste solution which is product produced from the reaction region after denature to a waste region by using the pneumatic actuator; and moving the remainder solution back to the reaction region by the pneumatic actuator.

REFERENCES:
patent: 5413708 (1995-05-01), Huse et al.
patent: 5482834 (1996-01-01), Gillespie
Krishnan et al. Microfabricated reaction and separation systems. Current Opinion in Biotechnology, vol. 12, p. 92-98, 2001.
Cheng et al. Preparation and hybridization analysis of DNA/RNA fromE.colion microfabricated bioelectronic chips. Nature Biotechnology, vol. 16, pp. 541-546, 1998.

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