Chemistry: molecular biology and microbiology – Apparatus – Means for separation or recovery of a microorganism from...
Patent
1995-08-03
1997-12-09
Warden, Jill
Chemistry: molecular biology and microbiology
Apparatus
Means for separation or recovery of a microorganism from...
210276, 210282, 210670, 422 70, 422101, 435 2, 435239, 436161, C12M 100, C12M 102
Patent
active
056959893
ABSTRACT:
A method and apparatus for separating target particles from a mixture of target and non-target particles. The apparatus includes a vessel having an inlet through which the mixture may be introduced and an outlet through which the fluid may exit, at least a portion of which is pliable; a bed of binding material disposed within the vessel, the binding material attracting the target particles such that the target particles may become bound thereto and being porous enough to allow the non-target particles to pass therethrough; and a screen disposed below the binding material for retaining the binding material in place. The pliable portion of the vessel permits the user to separate the target particles from the binding material by merely squeezing the pliable portion in such a manner as to cause relative movement in the binding material, thereby creating the necessary degree of agitation to cause the target particles to become dislodged from the binding material. The deformation of the pliable vessel can be accomplished by either the user physically squeezing the vessel with his/her hand or using an external apparatus that applies the necessary force to the vessel.
REFERENCES:
patent: 2063086 (1936-12-01), Fitz Gerald
patent: 3625652 (1971-12-01), Fujimoto et al.
patent: 3692493 (1972-09-01), Terasaki
patent: 3843324 (1974-10-01), Edelman et al.
patent: 3970518 (1976-07-01), Giaever
patent: 3986834 (1976-10-01), Steinbrink, Jr.
patent: 4230635 (1980-10-01), Senyei et al.
patent: 4647539 (1987-03-01), Bach
patent: 4787971 (1988-11-01), Donald
patent: 4839280 (1989-06-01), Banes
patent: 4871463 (1989-10-01), Taylor et al.
patent: 4904600 (1990-02-01), Ramp
patent: 4931400 (1990-06-01), Jitsukawa
patent: 5215926 (1993-06-01), Etchells, III et al.
patent: 5215927 (1993-06-01), Berenson et al.
patent: 5225353 (1993-07-01), Berenson et al.
patent: 5240856 (1993-08-01), Goffe et al.
patent: 5262334 (1993-11-01), Berenson et al.
patent: 5378624 (1995-01-01), Berenson et al.
patent: 5506130 (1996-04-01), Peterson et al.
Biorad Catalog p. 56, 85-86 (1988).
Miltenyi et al., "High Gradient Magnetic Cell Separation With MACS," Cytometry 11:231-238, 1990.
Berenson et al., "Positive selection of viable cell populations using avidin-biotin immunoadsorption," Journal of Immunological Methods 91: 11-19, 1986.
Basch et al., "Cell Separation Using Positive Immunoselective Techniques," Journal of Immunological Methods 56: 269-280, 1983.
Fredrickson and Basch "L3T4 Antigen Expression By Heompoietic Precusor Cells," Journal of Experimental Medicine 169: 1473-78, 1989.
Olson et al., "Dissociation Kinetics of Antigen-Antibody Interactions: Studies on a Panel of Anti-Albumin Monoclonal Antibodies," Molecular Immunology 26(2): 129-136, 1989.
Thomas et al, "Specific binding and release of cells from beads using cleavable tetrameric antibody complexes," Journal Immunological Methods 120(2): 221-231, 1989.
Wilchek and Bayer, "The Avidin-Biotin Complex in Bioanalytical Applications," Analytical Biochemistry 171:1-32, 1988.
Civin and Loken, "Cell Surface Antigens of Human Marrow Cells: Dissection of Hematopoietic Development Using Monoclonal Antibodies and Multiparameter Flow Cytometry," International Journal of Cell Cloning 5: 267-288, 1987.
Suzuki and Dale, "Biotinylated Erythrocytes: In Vivo Survial and In Vitro Recovery," Blood 70(3): 791-795, 1987.
Updyke and Nicolson, "Immunoaffinity Isolation of Membrane Antigens with Biotinylated Monoclonal Antibodies and Streptavidin--Agarose," Methods in Enzymology 121: 717-725, 1986.
Fuccillo, "Application of the Avidin-Biotin Technique in Microbiology," BioTechniques 3(6):494-501, 1985.
Wormmeester et al., "A Simple Method for Immunoselective Cell Separation with the Avidin-Biotin System," Journal of Immunological Methods 67: 389-394, 1984.
Kumar and Lykke, "Cell Separation: A Review," Pathology 16:53-62, 1984.
Bonnafous et al., "Cell Affinity Chromatography with Ligands Immobilized through Cleavable Mercury--Sulfur Bonds," Journal of Immunological Methods 58: 93-107, 1983.
Fong, "Solid-Phase Fractionation of Lymphoid Cells on Ligand-Coated Plastic Plates," Cell Separation: Methods and Selected Applications 2:203-219, 1983.
Lakow and Basch, "Positive Immunoselection Using Antibody-Enzyme Complexes," Journal of Immunological Methods 44: 135-151, 1981.
Wysocki and Sato, "Panning for lymphocytes: A method for cell selection," Proc. Natl. Acad. Sci. USA 75: 2844-2848, 1978.
Ghetie et al., "Separation of Cells by Affinity Chromatography on SpA-Sephrose 6MB," Journal of Immunological Methods 21: 133-141, 1978.
Wigzell, "Specific Affinity Fractionation of Lymphocytes Using Glass or Plastic Bead Columns," Scandinavian Journal of Immunology 5(Suppl. 5): 23-30, 1976.
Jasiewicz et al., "Selective Retieval of Biotin-Labeled Cells using Immobilized Avidin," Experimental Cell Research 100: 213-217, 1976.
Kiefer et al., "Separation of antigen-specific lymphocytes: A new general method of releasing cells bound to nylon mesh," European Journal of Immunology 5: 624-627, 1975.
Schlossman and Hudson, "Specific Purification of Lymphocyte Populations on a Digestible Immunoabsorbent," Journal of Immunology 110(1): 313-315, 1973.
Wigzell and Andersson, "Cell Separation on Antigen-Coated Columns: Elimination of High Rate Antibody-Forming Cells and Immunological Memory Cells," Journal of Experimental Medicine 129(1):23-36, 1969.
Edelman and Rutishauser, "Specific Fractionation and Manipulation of Cells with Chemically Derivatized Fibers and Surfaces," Methods in Enzymology 34: 195-225, 1974.
Norton and Williams, "Cell Sorting by Immunoaffinity Chromatography," Biotechniques 1: 96-100, 1983.
Goding, James W., "Affinity Chromatography Using Monoclonal Antibodies," Monoclonal Antibodies: Principles and Practice,2nd ed., Academic Press, Sydney, 1986, pp. 219-223.
Cell Affinity Chromatography: Principles & Methods, Pharmacia Fine Chemicals, 1980, pp. 8-18.
Klaus (ed.), Lymphocytes: A Practical Approach, IRL Press, Oxford, 1987, pp. 1,26.
Gorin, "Cryopreservation and Storage of Stem Cells," in Areman et al., Bone Marrow and Stem Cell Processing: A Manual of Current Techniques, F.A. Davis Co., Philadelphia, 1992 pp. 292-308.
Rose et al., (eds.), Manual of Clinical Laboratory Immunology, 3rd ed., American Society for Microbiology, Wash., D.C., 1986, p. 843.
Henry et al., "Nylon Wool," in Mishell et al., Selected Methods in Cellular Immunology, W.H. Freeman & Co., San Francisco, 1980, pp. 182-185.
CellPro, Inc.
Ludlow Jan M.
Warden Jill
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