Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Recovery or purification
Reexamination Certificate
2008-01-01
2008-01-01
Strzelecka, Teresa E. (Department: 1637)
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Recovery or purification
C536S023100
Reexamination Certificate
active
10527618
ABSTRACT:
Apparatus and methods are described for pharmaceutical grade manufacture extrachromosomal nucleic acids from cell lysates using flotation to separate and eliminate undesired insoluble cellular debris including chromosomal DNA from the lysates. A gas is introduced to controllably generate bubbles that reduce the density of the cell debris and create a buoyant flocculent phase that can be readily separated from, and thus provide, a substantially clarified fluid lysate phase that is enriched in extrachromosomal DNA but substantially depleted of cellular proteins and chromosomal DNA.
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Au-Yeung Kwok-Leung (Jacky)
Bussey Lee B.
Pande Suchira
Strzelecka Teresa E.
Valentis, Inc.
Wong Cabello Lutsch Rutherford & Brucculeri LLP
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