Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1988-06-06
1991-01-01
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
436514, 436518, 436531, 436807, 436169, 436162, 422 56, 422 58, 422 70, 422102, 422104, 422 8205, 422 8209, 422 8208, 210635, 210638, 2101983, C12Q 100, G01N 3353
Patent
active
049817850
ABSTRACT:
An apparatus and method for performing enzyme-linked immunological sandwich assays (ELISA) by affinity chromatography. Formation of the antibody-antigen-conjugate complex, and the development of a chromatophore indicator take place in a semi-automated, walk-away, fashion. The immunoassay apparatus comprises a chromatography tank, which further comprises a reaction compartment and a plurality of liquid solvent reservoirs, with a first-liquid reservoir being configured higher than a second-liquid reservoir. The apparatus allows automatic sequential and discrete movement of wash solution, followed by a color development solution, with unbound conjugate being substantially washed away from the immunological sandwich complex before the color development solution automatically comes into contact with the immunological complex.
REFERENCES:
patent: 3420708 (1969-01-01), Schaffer
patent: 4235601 (1980-11-01), Deutsch
patent: 4435504 (1984-03-01), Zuk
patent: 4446232 (1984-05-01), Liotta
patent: 4751060 (1988-06-01), Kratochwill
Cantor Frederick
Sisson Bradley L.
Ventrex Laboratories Inc.
Wax Robert A.
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