Apparatus and method for performing automated amplification of n

Heat exchange – With alarm – indicator – signal – register – recorder – test or... – Remotely controlled inspection means

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7386311, 236 46R, 422116, 436 50, F23N 520, G01N 3502

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active

050388525

ABSTRACT:
There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

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