Apparatus and method for optically analyzing a plurality of anal

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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422 64, 422 65, 422 67, 435 11, 435 16, 435 19, 435 24, 435 26, 435 28, G01N 3506, C12Q 160, C12Q 128

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active

046686177

ABSTRACT:
An apparatus and method for analyzing at least two analytical items in a sample solution in a single container, wherein a series of transparent containers supported on a rotatable disc pass over a sample-receiving position, a first reagent solution-adding position, a second reagent solution-adding position, an optical path of a photometer, a solution-discharging device and a washing device. In the sample-receiving position, a fixed volume of sample is introduced into one of the transparent containers. In the first reagent solution-adding position, the first reagent solution is introduced into the transparent container, whereby a reaction is allowed to proceed. Subsequently, the container is passed across an optical path, and absorbance of light by the reaction solution is measured, to provide first measurements. Thereafter, a second reagent solution is introduced into the transparent container at the second reagent solution-adding position, without removal of the first reaction solution, whereby a further reaction is allowed to proceed. Subsequently, the container is passed across an optical path, and absorbance of light by the resulting solution in the transparent container is measured, to provide second measurements. The first analytical item can be investigated from the first measurements, and the second analytical item can be investigated from differences between the first and second measurements.

REFERENCES:
patent: 4158545 (1979-06-01), Yamashita et al.
patent: 4276051 (1981-06-01), Ginsberg et al.
patent: 4313735 (1982-02-01), Yamashita et al.
patent: 4338279 (1982-07-01), Orimo et al.

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