Apparatus and method for nucleic acid isolation using supercriti

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 29, 4352831, 4352871, 4352872, 436527, 210600, 210634, C12Q 168, C12M 100, G01N 33552, B01D 2128

Patent

active

061106747

ABSTRACT:
A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

REFERENCES:
patent: 4749522 (1988-06-01), Kamarei
patent: 5059527 (1991-10-01), White et al.
patent: 5094753 (1992-03-01), Allington et al.
patent: 5306637 (1994-04-01), Lin et al.
patent: 5380826 (1995-01-01), Castor et al.
patent: 5487981 (1996-01-01), Nivens et al.
patent: 5587128 (1996-12-01), Wilding et al.
patent: 5645801 (1997-07-01), Bouma et al.
patent: 5656493 (1997-08-01), Mallis et al.
Yu-Li Tsai, Betty H. Olson, "Rapid Method for Direct Extraction of DNA from Soil and Sediments", Applied And Environmental Microbiology, Apr. 1991, p. 1070-1074.
More, Herrick, Silva, Ghoirse, Madsen, "Quantitative Cell Lysis of Indigenous Microorganisms and Rapid Extraction of Microbial DNA from Sediment", Applied And Environmental Microbiology, May 1994, p. 1572-1580.
Somerville, Knight, Straube, Colwell, "Simple, Rapid Method for Direct Isolation of Nucleic Acids form Aquatic Environments", Applied And Environmental Microbiology, May 1989, p. 548-554.
Steffan, Goksoyr, Bej, Atlas, "Recovery of DNA from Soils and Sediments", Applied And Environmental Microbiology, Dec. 1988, p. 2908-2915.
Ogram, Sayler, Barkay, "The extraction and purification of microbial DNA from sediments", Journal of Microbiological Methods 7, 1987, p. 57-66.
Picard, Ponsonnet, Paget, Nesme, Simonet, "Detection and Enumeration of Bacteria in Soil by Direct DNA Extraction and Polymerase Chain Reaction", Applied And Environmental Microbiology, Sep. 1992, p. 2717-2722.
Nili Grossman, Eliora Ron, "Membrane-Bound DNA From Escherichia Coli: Extraction By Freeze-Thaw-Lysozyme", FEBS Letters, Jul. 1975, vol. 54, No. 3, p. 327-329.
Bej, DiCesare, Haff, Atlas, "Detection of Escherichia coli and Shigella spp. in Water by Using the Polymerase Chain Reaction and Gene Probes for uid", Applied And Environmental Microbiology, Apr. 1991, p. 1013-1017.
Yu-Li Tsai, Betty Olson, "Rapid Method for Separation of Bacterial DNA from Humic Substances in Sediments for Polymerase Chain Reaction", Applied And Environmental Microbiology, Jul. 1992, p. 2292-2295.
Yu-Li Tsai, Carol Palmer, Louis Sangermano, "Detection of Escherichia coli in Sewage and Sludge by Polymerase Chain Reaction", Applied And Environmental Microbiology, Feb. 1993, p. 353-357.
Holben, Jansson, Chelm, Tiedje, "DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community", Applied And Environmental Microbiology, Mar. 1988, p. 703-711.
Johnson, Pieniazek, Griffin, Misener, Rose, "Development of a PCR Protocol for Sensitive Detection of Cryptosporidium Oocysts in Water Samples", Applied And Environmental Microbiology, Nov. 1995, p. 3849-3855.
Bej, Mahbubani, Dicesare, Atlas, "Polymerase Chain Reaction-Gene Probe Detection of Microorganisms by Using Filter-Concentrated Samples", Applied And Environmental Microbiology, Dec. 1991, p. 3529-3534.
Bej, Steffan, DiCesare, Haff, Atlas, "Detection of Coliform Bacteria in Water by Polymerase Chain Reaction and Gene Probes", Applied And Environmental Microbiology, Feb. 1990, p. 307-314.
Rong-Fu Wang, Allison Luneau, Wei-Wen Cao, Carl Cerniglia, "PCR Detection of Polycyclic Aromatic Hydrocarbon-Degrading Mycobacteria", Environ. Sci. Technol., 1996, p. 307-311.
Gary Jones, Peter Nichols, Philip Shaw, "Analysis of Microbial Sterols and Hopanoids", Chemical Methods in Prokaryotic Systematics, p. 163-195.
"Preparation of Genomic DNA from Bacteria", Basic Protocol, Unit 2.4, 1987.
"Recovery of DNA from glass fiber filters after exposure to supercritical fluid conditions".

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Apparatus and method for nucleic acid isolation using supercriti does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Apparatus and method for nucleic acid isolation using supercriti, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus and method for nucleic acid isolation using supercriti will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1248375

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.