Optics: measuring and testing – Blood analysis – Hemoglobin concentration
Patent
1990-12-04
1993-03-16
McGraw, Vincent P.
Optics: measuring and testing
Blood analysis
Hemoglobin concentration
356335, 356336, 356337, 356340, 356 73, 422 8205, 436164, 435808, G01N 3348
Patent
active
051949091
ABSTRACT:
An apparatus and method for measuring the volume and hemoglobin concentration of individual red blood cells in a whole blood sample provides for isovolumetrically sphering the red blood cells of the sample using a sphering reagent and forming a stream of individual, spaced-apart cells sheathed in an electrolytic liquid in a flow chamber. The red blood cells in the stream are passed through a light scattering channel in which the intensity of light scattered by each cell into a preselected angular interval in the forward direction is measured. Each cell in the stream is also passed through a resistance pulse sizing aperture for measuring the volume of the cell. The angular interval within which the intensity of scattered light intensity is measured is selected such that for each measured value of scattered light intensity corresponding to a red blood cell and value of the volume of the cell, as determined by the resistance pulse sizing, a unique value of hemoglobin concentration for the cell may be calculated over the entire physiological range of values of cell volume and cell hemoglobin concentration. The values of the cell volume and cell hemoglobin concentration are then used for deriving clinically useful statistical characteristics of the blood sample, such as the mean cell volume, the mean cell hemoglobin concentration, the width of the cell volume distribution and the width of the cell hemoglobin concentration distribution.
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Keesee, II LaCharles P.
McGraw Vincent P.
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