Apparatus and method for measuring the signal from a...

Details Apparatus and method for measuring the signal from a... Apparatus and method for measuring the signal from a...

2008-07-08

2008-07-08

Porta, David P. (Department: 2884)

Radiant energy

Invisible radiant energy responsive electric signalling

With or including a luminophor

C250S364000

Reexamination Certificate

active

07397036

ABSTRACT:
Apparatus and method for measuring the fluorescence of nanodrop liquid samples is described in which the sample is held by surface tension between two anvil surfaces. Each anvil surface has an embedded optical fiber with its end finished flush with the surface in the containment area wetted by the sample with the fiber in line. Sample excitation is provided from the side of the sample remote from the containment area. By selection of the fiber transmission numeric aperture the impact of exciting and ambient light on the measurement is minimized. A method of virtual filtering is taught in which any ambient or exciting light that does impinge on the measuring sensor is corrected by subtracting a scaled representation of the source from the measurement. The method and apparatus is capable of detecting 1 femptomole of sodium fluorescein in 1 microliter of TE buffer.

REFERENCES:
patent: 5422719 (1995-06-01), Goldstein
patent: 5500536 (1996-03-01), Nogami et al.
patent: 5678751 (1997-10-01), Buchanan et al.
patent: 5922285 (1999-07-01), Melendez et al.
patent: 6313471 (2001-11-01), Glebeler et al.
patent: 6574406 (2003-06-01), Ainslie et al.
patent: 6628382 (2003-09-01), Robertson
patent: 6809826 (2004-10-01), Robertson
patent: 6836332 (2004-12-01), Mosley et al.
patent: 2006/0109467 (2006-05-01), Evans
patent: 2006/0109468 (2006-05-01), Evans
patent: 2008/0028854 (2008-02-01), Evans
“Principles of Fluorescence Spectroscopy”, Joseph R. Lakewitz, 1999, Kluwer Academic/Plenum Publishers, 233 Spring St, New York, NY 10013, pp. 1-9.

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