Optics: measuring and testing – By shade or color – With color transmitting filter
Reexamination Certificate
2007-11-13
2010-12-14
Geisel, Kara E (Department: 2877)
Optics: measuring and testing
By shade or color
With color transmitting filter
Reexamination Certificate
active
07852479
ABSTRACT:
Apparatus and methods for measuring the fluorescence of large multi-cellular organisms in a sample of liquid includes a pumping mechanism, a fluorescence measuring device, a method of analyzing the measurements, and optionally, a sorting mechanism. The pumping mechanism transfers large multi-cellular organisms from a reservoir through a fluorescence-measuring device causing minimum physical damage and/or stress. The pressure differential driving the organisms from a sample container/reservoir through the measuring device can be derived from gravity, air pressure, or liquid pressure, or some combination of the three. The fluorescence can be measured in a cytometer using a light detector or imager. Generally the detection element will include a filter, isolating the wavelength of fluorescent emission. The illumination may be provided by a laser or by an LED, combined with the use of dichroic mirrors to allow multiple wavelength simultaneous illumination.
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Bales Jennifer L.
Geisel Kara E
Macheledt Bales LLP
University of Wyoming
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