Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...
Patent
1996-09-03
1999-03-30
Beisner, William H.
Chemistry: electrical and wave energy
Processes and products
Electrophoresis or electro-osmosis processes and electrolyte...
204603, G01N 2726, G01N 27447
Patent
active
058883630
ABSTRACT:
An apparatus and method for interfacing a capillary with a detector is disclosed. In particular, a capillary that is used in a capillary electrophoresis technique is interfaced with an off-column detector that is destructive of the sample or that will otherwise create adverse affects on the operations of the capillary electrophoresis. By way of example only, a nitrogen chemiluminescent detector using pyro-chemiluminescent techniques is discussed. An interface between the separation and detection systems is essential to prevent interference between the two systems. The interface apparatus and method are achieved by creating a closed connection between the two systems within which the pressure and sample flow rate can be controlled. Pressure control is achieved through the introduction and venting of gas into and from the integrated system. Interference between interfaced systems is then prevented by equalizing the pressure in the interface apparatus with the head pressure on the inlet end of the capillary. Also disclosed is a device for controlling the head pressure and sample flow within the capillary thereby enabling operation of an interfaced system at non-atmospheric pressure conditions.
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Abubaker Mohammad Amin
Bissell Michael G.
Fujinari Eugene M.
Petersen John R.
Antek Instruments, Inc.
Beisner William H.
Starsiak Jr. John S.
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