Apparatus and method for imaging a histological sample

Details Apparatus and method for imaging a histological sample Apparatus and method for imaging a histological sample

2006-01-31

2006-01-31

Evans, F. L. (Department: 2877)

Optics: measuring and testing

By dispersed light spectroscopy

With sample excitation

C356S036000, C250S459100, C435S040520

Reexamination Certificate

active

06992760

ABSTRACT:
A method of imaging a histological sample is provided which comprises stimulating autofluorescence in a sample using electromagnetic radiation of wavelength 750 nm. Detection of the autofluorescence is undertaken by a CCD camera, typically with an excitation filter of 510 nm disposed between the camera and the sample. The weak signal from autofluorescence is reinforced by manipulation of data acquired by the camera. Autofluorescence is detected from an upper face of the sample, an upper layer of the sample removed and a next face of the sample imaged. These steps are repeated to obtain information on autofluorescence throughout the sample. The method enables detection of features for virtually any thickness of slice, and in particular slices can range from 50 μm to 1 μm, with the method generally being used to analyse slices of thickness 5−1 μm. There is also provided a method of preparing a histological sample for use in the above method, and apparatus (10) for imaging autofluorescence in histological samples.

REFERENCES:
patent: 4497792 (1985-02-01), Gindler
patent: 4960330 (1990-10-01), Kerschmann
patent: 5682244 (1997-10-01), Barlow et al.
patent: 6372512 (2002-04-01), Kerschmann

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