Apparatus and method for gel casting and electrophoresis in a si

Chemistry: electrical and wave energy – Apparatus – Coating – forming or etching by sputtering

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2041828, 249161, G01N 2728, G01N 2726, B61D 5702

Patent

active

049542362

ABSTRACT:
Slab gels are cast in a gel enclosure which consists of a flat rectangular plate held against a larger plate by a flexible sealing sheet with a removable edge. The smaller plate is retained within raised edges along three sides of the larger plate and rests on a pair of shoulders along two of the raised edges. The sealing sheet spans all three raised edges and the smaller plate resting between them, thereby sealing three sides of the chamber between the plates, the width of the chamber being established by the shoulders. One edge of the sealing sheet is removable to expose the lower end of the gel slab to a buffer solution to permit the performance of an electrophoretic separation in the enclosure once the gel has been formed. A depression in the raised edge underlying the removable edge of the sealing sheet increases the access of a buffer solution to the lower end of the gel slab during electrophoresis.

REFERENCES:
patent: 3879280 (1975-04-01), Peterson et al.
patent: 4560459 (1985-12-01), Hoefer
patent: 4795541 (1989-01-01), Hurd et al.
Isolab, Inc. Literature on Electrophoresis Products.
Schleicher & Schuell Literature on S&S Profile Polyacrylamide Gels.
Jule, Inc. literature on Precast Gels.

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