Apparatus and method for gel casting

Details Apparatus and method for gel casting Apparatus and method for gel casting

1985-12-12

1988-11-15

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041801, G01N 2728, G01N 2726

Patent

active

047847387

ABSTRACT:
A gel used for electrophoresis is cast in a gel enclosure in which the gel will ultimately be used during the electrophoresis, by a novel method and using novel apparatus which provide for quick assembly and avoidance of leaks. The method and apparatus involve a wettable, preferably porous, membrane such as filter paper, which is thoroughly wetted with gel forming solution, then placed over the bottom opening of the gel enclosure and held in place until the gel on the membrane sets. Sealing of any cracks, gaps or irregularities along the rim of the opening is thereby achieved by the capillary action of the gel forming solution. The enclosure itself is then filled with gel forming solution. Once the latter has set, the membrane is removed.

REFERENCES:
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patent: 3751357 (1973-08-01), Rains
patent: 3773646 (1973-11-01), Mandle et al.
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patent: 3879280 (1975-04-01), Peterson et al.
patent: 4111784 (1978-09-01), Dahms
patent: 4290871 (1981-09-01), Hoefer et al.
patent: 4325796 (1982-04-01), Hoefer et al.

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