Radiant energy – Luminophor irradiation – With ultraviolet source
Patent
1992-08-25
1994-05-24
Dzierzynski, Paul M.
Radiant energy
Luminophor irradiation
With ultraviolet source
2504591, 356318, G01N 2164
Patent
active
053151224
ABSTRACT:
A flow cytometer measures fluorescence lifetimes by the phase shift of a reference signal and an emission from a fluorescent particle or cell in a flow chamber in order to identify the particle or cell. An acoustic optic modulator modulates laser light with a sinusoidal wave of a predetermined frequency to excite particles or cells. Detectors respond to emissions of individual particles or cells in the form of an output signal pulse at the predetermined frequency. Signals representative of fluorescence emitted and reference beam signals is measured. The particle is identified by the phase shift.
REFERENCES:
patent: 3855930 (1989-08-01), Chao et al.
patent: 4058732 (1977-11-01), Wieder
patent: 4198567 (1980-04-01), Eneroth et al.
patent: 4778593 (1988-10-01), Yamashita et al.
patent: 4910467 (1990-03-01), Leitch
Spencer et al, "Fluorescence Lifetimes" Annals of the NY Academy of Sciences p. 361 (1969).
"Microwave Systems Designer Handbook," Jul., 1987, vol. 17, p. 155.
"Radio Concepts," Ralph S. Carson, pp. 279-282.
Spencer, Richard Dale, 1942. Fluorescence Lifetimes: Theory, Instrumentation, and Application of Nanosecond Fluorometry. University of Illinois at Urbana-Champaign, Ph.D., 1970.
Spencer, Richard D. and Weber, Gregorio. Measurements of Subnanosecond Fluorescence Lifetimes with a Cross-Correlation Phase Fluorometer. University of Illinois at Urbana, Ill.
Berndt et al., "Phase-Modulation Fluorometry Using a Frequency-Doubled Pulsed Laser Diode Light Source," Rev. Sci. Instrum., 61(7), p. 1816 (1990).
Vo-Dinh et al., "Phase-Resolved Fiber-Optics Fluoroimmunosensor," Applied Spectroscopy, 44(1), p. 128 (Jan. 1990).
Ladasky, Jr. John J.
Pinsky Bertram G.
Becton Dickinson and Company
Dzierzynski Paul M.
Hanig Richard
Schwarz Michael G.
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