Apparatus and method for determining the concentration in a samp

Optics: measuring and testing – By shade or color – Fluid color transmission examination

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73 231, G01N 2148, G01N 3108

Patent

active

041992601

ABSTRACT:
Apparatus and method for determining the concentration of a constituent in a sample using chromatographic and optical measuring techniques. A sample containing a constituent to be analyzed is fractionated by a chromatographic column. A light beam is passed through the constituent fraction in the column, and the light absorbance (or transmittance) is measured. The fluid carrier (or solvent) eluting through the column will generally increase the transmissivity of the light passing through the column due to a difference between the indices of refraction of the carrier fluid and the chromatographic packing material. Such increased transmissivity will generally offset any decrease in the transmittance due to trace amounts of the constituent in the sample. The optical measurement is compensated to nullify such increased transmissivity, so as to allow measurement of such trace amounts of constituents.

REFERENCES:
patent: 1760209 (1930-05-01), Pfeiffer

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