Optics: measuring and testing – By light interference – Holography
Reexamination Certificate
2006-10-17
2008-12-02
Lyons, Michael A (Department: 2877)
Optics: measuring and testing
By light interference
Holography
Reexamination Certificate
active
07460240
ABSTRACT:
The present invention utilizes spatially modulated optical force microscopy (SMOFM) with single beam optical force probing capability or with a holographic optical trapping system capable of multi-beam optical force probing coupled to a microscope objective, to generate a probe beam(s) as a force probe to perturb the object that is adhered or resting on a surface, so that deformations of the object may subsequently be quantified. This quantification is performed by imaging a sequence of four phase shifted replicas of the image using a computer-controlled spatial light modulator, and calculating the pixel by pixel optical path-length using existing algorithms. The change in optical path lengths, and consequently the viscoelastic or elastic response elicited, is an indication of damage or disease when the objects are cells. In another embodiment, the optical deformability of the cells may be measured and correlated with measurements of cytoskeletal/structural protein expression.
REFERENCES:
patent: 5689109 (1997-11-01), Schutze
patent: 6055106 (2000-04-01), Grier et al.
patent: 6416190 (2002-07-01), Grier et al.
patent: 2004/0085540 (2004-05-01), Lapotko et al.
Motility of live cancer cell quantified by Fourier phase microscopy, Popescu et al, SPIE-OSA vol. 5864, Aug. 2005, pp. 1-3.
Guck, J., et al., “Optical Deformability as an Inherent Cell Marker for Testing Malignant Transformation and Metastatic Competence”, Biophysical Journal, 88:3689-98, 2005.
Guck, J., et al., “The Optical Stretcher: A Novel Laser Tool to Micromanipulate Cells”, Biophysical Journal, 2001, 81:767-84.
Lekka, M., et al., “Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy”, European Biophysics Journal, 1999, 28:312-6.
Popescu, G., et al., “Fourier phase microscopy for investigation of biological structures and dynamics”, Optics Letters, 2004, 29(21):2503-5.
Dufresne, E.R., et al. “Computer-Generated Holographic Optical Tweezers Arrays”, Dept. of Physics et al., Oct. 27, 2000, 8.
Janmey, P.A., et al. “Viscoelastic Properties of Vimentin Compared with Other Filamentous Biopolymer Networks”, The Journal of Cell Biology, 1991, 113(1), 155-160.
Jenmey, P.A., et al. “Dealing with mechanics: mechanisms of force transduction in cells”, Trends in Biochemical Sciences, 2004, 29(7), 364-370.
Voros, Janos, “The Density and Refractive Index of Adsorbing Protein Layers”, Biophysical Journal, 2004, 87, 553-561.
Kang, Y., et al. Epithelial-Mesenchymal Transitions: Twist in Development and Metastasis, Cell, 2004, 118, 277-279.
Thiery, Jean Paul, Epithelial-Mesenchymal Transitions in Tumour Progression, Nature Publishing Group, 2002, 2, 442-454.
Ghosh, S., et al. “Loss of Adhesion-Regulated Proteinase Production Is Correlated with Invasive . . . Cell Carcinoma”, American Cancer Society, 2002, 2524-2533.
Yang, J., et al. “Twist, a Master Regulator of Morphogenesis, Plays an Essential Role in Tumor Metastasis”, Cell, 2004, 117, 927-939.
Paszek, M.J., et al. “The Tension Mounts: Mechanics Meets Morphogenesis and Malignancy”, Journal of Mammary Gland Biology and neoplasia, 2004, 9(4), 325-342.
MacKintosh, F.C., et al. “Elasticity of Semiflexible Biopolymer Networks”, Physical Review Letters, 1995, 75(24), 4425-4429.
Akerman & Senterfitt
Arryx, Inc.
Edwards, Esq. Jean C.
Lyons Michael A
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