Apparatus and method for culturing embryonic stem cells

Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus

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4352402, 43524023, 435285, 435287, 435297, C12M 300, C12M 122, C12M 304, C12N 526

Patent

active

054496209

ABSTRACT:
Apparatus and method for culturing at least two different cell types in close proximity are provided. A solid support having at least one depression wherein a controlled concentration of a selected cell type can be layered is employed in the invention.

REFERENCES:
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patent: 4894343 (1990-01-01), Tanaka et al.
patent: 5157024 (1992-10-01), Gordon
Wood et al., Simple and efficient production of enbryonic stem cell-embryo chimeras by coculture, Proc. Natl. Acad. Sci. USA 1993, 90, 4582-4585.
Nagy et al., Derivation of completely cell culture-derived mice from early-passage embryonic stem cells, Proc. Natl. Sci. USA 1993, 90, 8424-8428.
Wood et al., Non-injection methods for the production of embryonic stem cell-embryo chimaeras, Nature 1993, 365, 87-89.

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