Coating apparatus – With vacuum or fluid pressure chamber – With means to apply electrical and/or radiant energy to work...
Patent
1984-11-30
1986-02-04
Lusignan, Michael R.
Coating apparatus
With vacuum or fluid pressure chamber
With means to apply electrical and/or radiant energy to work...
34 92, 422131, 4221863, F26B 1330, C23C 1400, B01J 1908, B01J 1912
Patent
active
045678472
ABSTRACT:
This invention relates to apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
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Board of Regents , The University of Texas System
Lusignan Michael R.
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