Refrigeration – With illuminating or radiant energy means
Patent
1987-04-13
1988-05-24
Bennet, Henry A.
Refrigeration
With illuminating or radiant energy means
34 92, 118 501, 422131, 62514R, F25D 2300
Patent
active
047457716
ABSTRACT:
This invention relates to sample holder for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a thermally conductive sample holder at a temperature of less than - 140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
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Linner John G.
Livesey Stephen A.
Bennet Henry A.
Board of Regents , The University of Texas System
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