Refrigeration – Processes – Treating an article
Patent
1985-08-29
1987-06-30
Lusignan, Michael R.
Refrigeration
Processes
Treating an article
34 5, 422 1, 422 22, F25D 1702, F26B 506, F26B 510, A61L 200
Patent
active
046760709
ABSTRACT:
This invention relates to method and apparatus for the cryopreparation of biological tissue samples for ultrastructural analysis. The use of the apparatus comprises vitrifying a biological tissue sample under cryogenic temperature conditions and ultra low vacuum conditions. The depressurized, vitrified tissue sample is brought to equilibrium in a sample holder at a temperature of less than -140.degree. C. The tissue sample is then dehydrated while maintained in a state of thermal equilibrium. After reaching equilibrium the tissue sample is optionally infiltrated with a degassed resin followed by a polymerization of the resin to form an embedded tissue sample.
REFERENCES:
patent: 3679450 (1972-07-01), Beightol
patent: 3942519 (1976-03-01), Shock
patent: 4055904 (1977-11-01), Horne
patent: 4056855 (1977-11-01), Kelman
patent: 4100158 (1978-07-01), Hydes et al.
patent: 4120991 (1978-10-01), Ornstein et al.
patent: 4197658 (1980-04-01), Fraser
patent: 4232453 (1980-11-01), Edelmann
patent: 4248821 (1981-02-01), Van Dellen
patent: 4266111 (1981-05-01), Trillwood
patent: 4269713 (1981-05-01), Yamashita et al.
patent: 4278623 (1981-07-01), Niegisch
patent: 4278701 (1981-07-01), von Hagens
patent: 4331591 (1982-05-01), Baylis
patent: 4336691 (1982-06-01), Burstein et al.
patent: 4337240 (1982-06-01), Saklad
patent: 4347671 (1982-09-01), Dias et al.
patent: 4379003 (1983-04-01), Robbins et al.
patent: 4423600 (1984-01-01), McKenna
patent: 4449305 (1984-05-01), Baron et al.
patent: 4559298 (1985-12-01), Fahy
Terracio, Louis et al, Freezing and Drying of Biological Tissues for Electron Microscopy, pp. 1021-1028, 1981.
Sleytr, U. B. et al, Understanding the Artefact Problem in Freeze-Fracture Replication: A Review, 1982.
Smolin, G. et al, The Cornea, Scientific Foundations and Clinical Practice, 1983.
Casey, T. et al, Corneal Grafting, Principles and Practice, 1984.
Lusignan Michael R.
The Board of Regents, The University of Texas
LandOfFree
Apparatus and method for cryopreparing biological tissue does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Apparatus and method for cryopreparing biological tissue, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Apparatus and method for cryopreparing biological tissue will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-440787