Apparatus and method for crosslinking to fix tissue or crosslink

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

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424423, 424520, 435 4052, 435174, 435177, 435325, 435366, 530402, 530810, 530812, A01N 102, A61F 200, G01N 130, C12N 1102

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059586699

ABSTRACT:
Crosslinking to prepare fixed tissue or to crosslink modification molecules to tissue is carried out with an apparatus and method wherein diffusion through a semipermeable is used to provide a selected molecular weight distribution of oligomers of a crosslinking compound that polymerizes spontaneously in solution to produce oligomers such as glutaraldehyde. The membrane has a molecular weight exclusion limit that prevents oligomers above a certain molecular weight from passing. Tissues treated with the size selected oligomers have improved properties. In the apparatus, the membrane separates a solution containing the crosslinking compound and oligomers from a solution in which crosslinking is carried out. The selected molecular weight distribution of oligomers diffuses through the membrane into the solution where tissue is crosslinked or a beneficial molecule is crosslinked to tissue.

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