Apparatus and method for cell analysis

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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Reexamination Certificate

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07488574

ABSTRACT:
A cell analysis apparatus includes a test sample preparation part for preparing a test sample by treating an analyte with a dye for staining an intracellular substance of cells in the analyte, a detector for detecting scattered light and fluorescence from each cell in the test sample, and a controller for identifying living mononuclear cells based on the scattered light and the fluorescence detected by the detector. A cell analysis method includes preparing a test sample by treating the analyte with a dye for staining an intracellular substance of cells in the analyte; detecting scattered light and fluorescence from each cell in the test sample; and identifying living mononuclear cells based on the scattered light and the fluorescence detected.

REFERENCES:
patent: 5434081 (1995-07-01), Maekawa
patent: 5656499 (1997-08-01), Chupp et al.
patent: 2002-148261 (2002-05-01), None
Bochner BS et al (1997) Studies of cell adhesion and flow cytometric analyses of degranulation, surface phenotype, and viability using human eosinophils, basophils, and mast cells. Methods, vol. 13, pp. 61-68.
Keeney, M. et al., “Single Platform Flow Cytometric Absolute CD34 + Cell Counts Based on the ISHAGE Guidelines”,Cytometry(Communications in Clinical Cytometry), vol. 34, issue 2, 1998, pp. 61-70.

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