Radiant energy – Calibration or standardization methods
Patent
1993-11-22
1995-05-09
Fields, Carolyn E.
Radiant energy
Calibration or standardization methods
2504581, 356243, G01N 2164
Patent
active
054142580
ABSTRACT:
The response of a fluorescence detector is calibrated using apparatus and methods for both static and dynamic calibrations. The apparatus and methods are especially suited for portable use in field calibration of fluorescence detectors of authentication systems that use non-visible light. The calibration apparatus incorporates standard target elements (20) comprising predetermined fluorescent substances with known emission spectra, and either non-fluorescent substances or fluorescent substances with other known emission spectra. The substances are either mixed in predetermined proportions, or one substance is arranged in a predetermined pattern (170), such as a bar code, on the other substances. An aperture (30) exposes a predetermined surface area of a standard target element to the view of the fluorescence detector. The distance of the standard target element from the optical front end (70) of the fluorescence detector to be calibrated is adjustable (50). For dynamic calibration, the standard target element is moved from one position to another in the apparatus in a predetermined time. Multiple standard target elements are substituted individually into the apparatus or are carried together on a carrier (80) that is indexed to present each standard target element in turn. Additional light can be admitted to the apparatus through a port (200) to simulate the ambient light encountered in normal use of the fluorescence detector. Standardized quick-disconnect couplings (220, 230) provide for easy exchange of the calibration apparatus among various fluorescence detectors.
REFERENCES:
patent: 4302678 (1981-11-01), Schiffert
patent: 4662745 (1987-05-01), Zupanick et al.
patent: 5089709 (1992-02-01), Chadwick
patent: 5093234 (1992-03-01), Schwartz
patent: 5125747 (1992-06-01), Sayegh et al.
West et al., "Practical Standards for UV Absorption and Fluorescence Spectrophotometry--Developments in Photophysical Instrumentation Part 3", American Laboratory, 9(3), Mar. 1977, pp. 37-52.
Angstrom Technologies, Inc.
Fields Carolyn E.
Touw Theodore
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