Apparatus and method for applying a laser beam through a microsc

Details Apparatus and method for applying a laser beam through a microsc Apparatus and method for applying a laser beam through a microsc

1992-02-27

1994-03-22

Henry, Jon W.

Optical: systems and elements

Compound lens system

Microscope

359385, 359202, 2191218, 4351731, 4352402, G02B 2106, G02B 2610, C12N 510, C12N 1300

Patent

active

052969630

ABSTRACT:
An inverted microscope is adapted so that a specimen thereon e.g. cells can be irradiated by a laser beam. The laser beam is guided from a laser source by a series of adjustably movable reflectors which introduce it into the microscope optical pathway at a parallel-beam region thereof, and through the objective lens of the microscope. A point to be irradiated can be selected by moving the reflectors which may be galvanometrically-movable. The laser beam can be focused together with the microscope image. The movable reflectors are kept within 200 mm behind the objective lens principal plane (PP) to ensure that the laser can be applied all over the microscope viewing field.

REFERENCES:
patent: 3705755 (1972-12-01), Baer
patent: 4204748 (1980-05-01), Yonekubo
patent: 4515447 (1985-05-01), Weimer et al.
patent: 5013660 (1991-05-01), Kasuya et al.
R. Weigand et al, "Laser-Induced Fusion . . . ", Jour. of Cell Science, vol. 88, 1987, pp. 145-149.

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