Apparatus and method for analyzing cells in urine

Details Apparatus and method for analyzing cells in urine Apparatus and method for analyzing cells in urine

1992-05-13

1994-06-28

Warden, Robert J.

Optics: measuring and testing

For optical fiber or waveguide inspection

356336, 356338, 356417, 209581, 209582, 209939, G01N 1502, G01N 2147, G01N 2164

Patent

active

053251687

ABSTRACT:
A method and apparatus are provided for analyzing cells in urine. A constricted zone through which various cells contained in a urine specimen flow in single file is irradiated with light having a light beam width in the direction of cell flow of between 1 .mu.m to 20 .mu.m. Scattered light and fluorescent light emitted by the cells is converted into scattered-light intensity data and fluorescent light intensity data. Preferably, the individual cells have been stained with a stain such that DNA will specifically emit fluorescence. Cell diameter data is also generated from the detected scattered light. Cell judgment values are stored in a memory device, and the cells in the urine specimen are classified based upon the cell judgment values.

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J. A. Steinkamp, "Flow Cytometry", Review of Scientific Instruments, vol. 55, No. 9, Sep. 1984, New York, pp. 1375-1400.

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