Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1993-04-22
1994-01-18
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, 435 6, 435287, 436 43, 935 77, G01N 2726, G01N 27447
Patent
active
052797210
ABSTRACT:
An automated electrophoresis and transfer apparatus for separating macromolecules in accordance with their molecular weight and transferring the same to a transfer membrane includes a housing having a perforated intermediate horizontal partition defining an upper reservoir and a lower chamber. A pair of spaced electrodes are provided for generating a horizontal electrical field. A pump is connected between a plurality of containers and the reservoir for sequentially pumping the chemical solutions within the container into the reservoir. A transfer membrane is positioned over the partition and an impermeable sheet is interposed between the membrane and the gel member and withdrawn prior to the transfer operation. A pump provides a small over pressure within the lower chamber which in cooperation with the impermeable sheet substantially prevents chemical solutions from penetrating the transfer membrane prior to the transfer operation. A vacuum pump provides a low pressure within the lower chamber and a pair of vertically spaced electrodes provides a vertical electrical field across the gel member and transfer membrane during the transfer operation.
REFERENCES:
patent: 4726889 (1988-02-01), Love et al.
patent: 4911816 (1990-03-01), Love et al.
patent: 5188963 (1993-02-01), Stapleton
R. K. Wilson et al. "Automation of Dideoxynucleotide DNA Sequencing Reactions Using a Robotic Workstation." Biotechniques, vol. 6 No. 8 (1988) 776-785.
Jackson Harold L.
Niebling John
Starsiak Jr. John S.
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