Apparatus and method for amplifying and detecting target nucleic

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Sample mechanical transport means in or for automated...

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422 67, 422 8205, 422104, 422109, 422119, 435290, 435291, 435310, 435316, 219385, 219390, 219521, 219535, C12P 1734, C12M 140

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054158397

ABSTRACT:
Methods, devices, apparatus and kits for amplifying and detecting nucleic acid are provided. The apparatus is a two-tier thermal cycling device that operates in conjunction with a reaction/detection unit. A sample is loaded into a reaction chamber of the device which is then mated with a detection chamber to form the reaction detection unit. A first heating element of the thermal cycling apparatus applies a desired temperature to the reaction/detection device to amplify target nucleic acid in the sample. The reaction mixture is then transferred to the detection chamber by the second heating element and amplified target nucleic acid is immobilized on a support in the detection chamber. A detection system associated with the apparatus detects and analyzes the immobilized amplified nucleic acid target.

REFERENCES:
patent: 4312835 (1982-01-01), Zoltan et al.
patent: 4727032 (1988-02-01), Baisch et al.
patent: 4865986 (1989-09-01), Coy et al.
patent: 5229297 (1993-07-01), Schnipelsky et al.
patent: 5270183 (1993-12-01), Corbett et al.

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