Apparatus, a method and software for analyzing a cell image

Image analysis – Applications – Biomedical applications

Reexamination Certificate

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C382S128000

Reexamination Certificate

active

08086016

ABSTRACT:
A cell image analysis apparatus and method for judging, quickly and with good reproducibility, the presence or absence of a membrane translocation reaction occurring in a cell owing to application of an arbitrary stimulus to the cell using a fluorescence microscope. The inventive apparatus has: an element acquiring as an image data a fluorescence microscopic image of a cell after the application of the stimulus; an element determining a region occupied by the cell in the fluorescence image of the cell; an element determining a contour line of the cell having a width of a predetermined number of pixels from a periphery of the region occupied by the cell; and an element judging the presence or absence of the membrane translocation reaction based on a brightness value on the contour line of the cell and a brightness value in an inside of the contour line of the cell.

REFERENCES:
patent: 5891646 (1999-04-01), Barak et al.
patent: 7279338 (2007-10-01), Kim et al.
patent: 2004-054347 (2004-02-01), None
Hundle, Bhupinder et al., “Overexpression of ∈-Protein Kinase C Enhances Nerve Growth Factor-induced Phosphorylation of Mitogen-activated Protein Kinases and Neurite Outgrowth”, The Journal of Biological Chemistry (1995), pp. 30134-30140, vol. 270, No. 50, the American Society for Biochemistry and Molecular Biology, Inc.

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