Image analysis – Applications – Biomedical applications
Reexamination Certificate
2009-02-05
2011-12-27
Mis, David (Department: 2817)
Image analysis
Applications
Biomedical applications
C382S128000
Reexamination Certificate
active
08086016
ABSTRACT:
A cell image analysis apparatus and method for judging, quickly and with good reproducibility, the presence or absence of a membrane translocation reaction occurring in a cell owing to application of an arbitrary stimulus to the cell using a fluorescence microscope. The inventive apparatus has: an element acquiring as an image data a fluorescence microscopic image of a cell after the application of the stimulus; an element determining a region occupied by the cell in the fluorescence image of the cell; an element determining a contour line of the cell having a width of a predetermined number of pixels from a periphery of the region occupied by the cell; and an element judging the presence or absence of the membrane translocation reaction based on a brightness value on the contour line of the cell and a brightness value in an inside of the contour line of the cell.
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Hundle, Bhupinder et al., “Overexpression of ∈-Protein Kinase C Enhances Nerve Growth Factor-induced Phosphorylation of Mitogen-activated Protein Kinases and Neurite Outgrowth”, The Journal of Biological Chemistry (1995), pp. 30134-30140, vol. 270, No. 50, the American Society for Biochemistry and Molecular Biology, Inc.
Kobayashi Tamiyo
Matsuo Yuichiro
Takagi Kosuke
Mis David
Olympus Corporation
Scully , Scott, Murphy & Presser, P.C.
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