Apoptosis gene EI24, compositions, and methods of use

Details Apoptosis gene EI24, compositions, and methods of use Apoptosis gene EI24, compositions, and methods of use

1996-03-21

1998-12-01

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 435914, 4353201, 435325, 536 235, C12Q 168, C12P 2100, C12N 510, C07H 2104

Patent

active

058436596

ABSTRACT:
Disclosed is the isolation and characterization of EI24, a novel gene whose 2.4 kb mRNA is induced following etoposide treatment. Induction of EI24 mRNA by etoposide required expression of wild-type p53. Overexpression of functional p53 was sufficient to induce expression of the EI24 mRNA. The EI24 mRNA was also induced in a p53-dependent manner by ionizing irradiation of primary murine thymocytes. The invention is thus directed to an isolated EI24 protein, nucleotide sequences coding for and regulating expression of the protein, antibodies directed against the protein, and recombinant vectors and host cells containing the genetic sequences coding for and regulating the expression of the protein sequence. The invention is also directed to genomic DNA, cDNA, and RNA encoding the EI24 protein sequence and to corresponding antisense RNA sequences. Antibodies can be used to detect EI24 in biological specimens, including, for example, human tissue samples. The present invention is further directed to methods of treating degenerative disorders characterized in inappropriate cell proliferation or inappropriate cell death. The present invention is further directed to methods for diagnosing degenerative disorders characterized in inappropriate cell proliferation or inappropriate cell death, as well as methods for monitoring the progress of such degenerative disorders.

REFERENCES:
Friedlander et al. A mutant p53 that discriminates between p53-responsive genes cannot induce apoptosis. Mol and Cell. Biol. vol. 16(9):4961-4971, Sep. 1996.
Ludwig et al. Differential activation of target cellular promoters by p53 mutants with impaired apoptotic function. Mol and Cell. Biol. Vol. 16(9):4951-4960, Sep. 1996.

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