Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1992-03-20
1994-08-23
Wax, Robert A.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
435 691, 435 713, 4351723, 4352523, 43525233, 4353201, 536 232, 935 10, 935 14, 935 29, 935 56, 935 72, 935 73, C12N 912, C12N 1554, C12N 1570
Patent
active
053407326
ABSTRACT:
An antiviral protein having an amino acid sequence represented by sequence No. 8 of a Sequence Listing is disclosed. The protein is obtained such that cysteine residues in the basic protein (MAP) obtained from Mirabilis jalapa and having antiviral activity are substituted with serine residues. More specifically, a MAP gene in which codons encoding cysteines are substituted with codons encoding serines is prepared, and this gene is integrated in a MAP secretion vector. This vector is introduced into a host to express the gene, thereby obtaining the antiviral protein. This protein retains MAP advantages as a toxic protein and has higher protein synthesis inhibition activity than that of MAP, and almost equal to that of a lysine A chain.
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C. N. Pace (1990) Tibs Trends in Biochemical Sciences, 15:14-17.
Habuka Noriyuki
Matsumoto Takashi
Miyano Masashi
Noma Masana
Japan Tobacco Inc.
Prouty Rebecca
Wax Robert A.
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