Antiuracil monoclonal antibody

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S006120, C435S007230, C435S007400, C435S007930, C435S070210, C435S452000, C435S333000, C436S518000, C436S548000, C436S822000, C530S388210, C530S807000

Reexamination Certificate

active

06927035

ABSTRACT:
A monoclonal antibody which reacts strongly with uracil and thymine but scarcely with N-carbamyl-β-alanine; a hybridoma producing this monoclonal antibody; a method of immunochemically assaying uracil or thymine characterized by using the above-described monoclonal antibody; and diagnostics for DPD deficiency containing the above monoclonal antibody. Because of high sensitivity and specific reaction with uracil and thymine, the above-described monoclonal antibody enables convenient, quick, and selective assaying of uracil and thymine in a sample. The antibody is useful in screening patients with DPD deficient cancer with contraindication to the administration of pyrimidine fluoride-based antitumor agents.

REFERENCES:
patent: 62-299765 (1987-12-01), None
patent: WO 99/20748 (1999-04-01), None
Honda et al., 2002. Development and characterization of a monoclonal antibody with cross-reactivity towards uracil and thymine, and its potential use in screening patients treated with 5-fluorouracil for possible risks. Clinica Chimia Acta 322: 59-66.
Hellstrom et al., 1985. In Monoclonal Antibodies for Cancer Detection and Therapy (Baldwin et al., eds.) Academic Press, London, p. 20.
Alarcon-Segovia et al., 1976. Immunochemical characterization of the anti-RNA antibodies found in scleroderma and systemic lupus erythematosus. II. Reactivity with HSA-coupled, uridine-containing, monophosphoric ribodinucleotides, Immunology 30: 413-41.
Uhlig et al., 1989. Monoclonal autoantibodies derived from multiple sclerosis patients and control persons and their reactivities with antigens of the central nervous system. Autoimmunity 5: 87-99.
Y. Tsutsui, et al., Database Biosis ‘Online!’ Biosciences Information Service, 1 page, XP-002205201, “Immuno Fluorescent Detection of Thermolability of Chromatin In-Situ During the Cell Cycle Using Anti Thymine Antibodies”, 1981.
D. Alarcon-Segovia, et al., The Lancet, vol. 15, pp. 363-365, XP-002915800, “Uracil-Specific Anti-R.N.A. Antibodies in Scleroderma”, Feb. 15, 1975.
Anita A. Piper, et al., Biochimica et Biophysica Acta, vol. 633, pp. 400-409, “The Activities of Thymidine Metabolishing Enzymes During the Cell Cycle of a Human Lymphocyte Cell Line LAZ-07 Synchronised by Centrifugal Elutriation”, 1980.
Albert H. Van Gennip, et al., Advances in Experimental Medicine and Biology, Purine and Pyrimidine Metabolism in Man VI, vol. 253A, pp. 111-118,“Comparative Study of Thymine and Uracil Metabolism in Healthy Persons and in a Patient with Dihydropyrimidine Dehydrogenase Deficiency”, 1989.
Satoshi Sumi, et al., Journal of Chromatography B, vol. 672, pp. 233-239, “Automated Screening System for Purine and Pyrimidine Metabolism Disorders Using High-Performance Liquid Chromatography”, 1995.

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