Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1999-06-14
2000-07-25
Witz, Jean C.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530322, 530395, A61K 3816, A61K 3814, C07K 1400
Patent
active
060936940
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention relates to a substance having an antitumor activity and, an antitumor composition and a health food, comprising the same. More particularly, the present invention relates to a substance having an antitumor activity, which substance is obtainable by collecting the extract residues with ethanol from the fruit body of Agaricus blazei Murill belonging to the genus Agaricus, degrading the residues with an acid and then purifying the acid-degraded product. The present invention further relates to an antitumor composition and a health food comprising such an antitumor-active substance.
BACKGROUND ART
Agaricus blazei Murill belonging to the genus Agaricus and also called himemaitsutake mushroom is a kind of mushrooms that grows wild mainly in the mountainous region of the southeastern Brazil, Sao Paulo. The natives have long been using Agaricus blazei Murill as an edible mushroom.
In these years, Agaricus blazei Murill has become widely cultured also in Japan to make its extracts available for the treatment of diabetes and hypertension.
Various investigations have been made to explore substances having an antitumor activity. It is reported that polysaccharides with an antitumor activity are obtained, for example, by extracting the fruit body or mycelium of Agaricus blazei Murill with an aqueous medium (Japanese Patent KOKAI (Laid-Open) Nos. 55-74797, 64-67194, 64-67195, 55-108292, etc.). It is also reported that a nucleic acid component having an antitumor activity is extracted from the fruit body of himematsutake mushroom (Japanese Patent KOKAI (Laid-Open) No. 64-66127). These substances having an antitumor activity are all extracted from the components soluble in an aqueous medium or soluble in hot water.
On the other hand, Japanese Patent Application KOKAI No. 2-78630 discloses that a proteoglycan having an antitumor activity was isolated from the extract residue of the Agaricus blazei Murill fruit body with hot water. More specifically, it is reported that the Agaricus blazei Murill fruit body was extracted with hot water to remove water-soluble components, the resulting residue was further extracted with hot 1% ammonium oxalate aqueous solution; then the extract residue gave the proteoglycan having an antitumor activity.
The substances mentioned above are obtained either from the components soluble in an aqueous medium or soluble in hot water, or from the extract residue with hot water. Thus these substances are all derived from the components insoluble in hot 1% ammonium oxalate aqueous solution.
On the other hand, the present inventors found that a substance having an antitumor activity was obtained from the extract residue of the Agaricus blazei Murill fruit body with hot water followed by further extraction with hot 1% ammonium oxalate aqueous solution. Japanese Patent Application No. 4-160924 (Japanese Patent KOKAI (Laid-Open) No. 6-9423) is directed to the substance.
However, this substance is not satisfactory for the treatment of solid tumor since its antitumor activity is not sufficiently potent.
Either the above substance obtained from the components of the Agaricus blazei Murill fruit body soluble in an aqueous medium or soluble in hot water or the substance insoluble in hot 1% ammonium oxalate aqueous solution which is obtained from the extract residue with hot water is yet insufficient in their antitumor activity.
DESCRIPTION OF THE INVENTION
The present inventors have found that a substance having a potent antitumor activity can be obtained by treating the Agaricus blazei Murill fruit body with hot ethanol, collecting the extract residues, degrading the residues with an acid, preferably after extracting with ammonium oxalate, or directly without ammonium oxalate extraction, and then purifying the acid degradation product. The inventors have further found that the substance is useful as an active ingredient of an antitumor composition and of a health food. The present invention has thus been accomplished.
The present invention relates to a substa
REFERENCES:
Kawagishi et al. Fractionation and Antitumor Activity of the Water-Insoluble Residue of Agaricus blazei Fruiting Bodies: Carbohydrate Research, vol. 186 pp. 267-273, 1989.
Ebina Takusaburo
Fujimiya Yoshiaki
Patten Patricia
Sumitomo Forestry Co., Ltd.
Witz Jean C.
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