Antisense oligonucleotides to the RAS gene

Details Antisense oligonucleotides to the RAS gene Antisense oligonucleotides to the RAS gene

1992-12-14

1996-12-10

LeGuyader, John L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 913, 4351721, 536 231, 536 241, 536 245, C12Q 168, C12N 1510

Patent

active

055829724

ABSTRACT:
Methods are provided for preparing antisense oligonucleotides which take advantage of RNA secondary and tertiary structure and for preparing antisense oligonucleotides which specifically hybridize to regions of RNA secondary and tertiary structure by comparing the affinity of the oligonucleotide for a structured RNA target to the affinity of the oligonucleotide for a length-matched oligonucleotide complement, and selecting an oligonucleotide having an affinity for the structured target which is not less than one thousandth of its affinity for the length-matched oligonucleotide complement. Oligonucleotides are also disclosed which are specifically hybridizable with regions of H-ras RNA having secondary and tertiary structure.

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