Antisense oligonucleotides to the RAS gene

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 913, 4351721, 536 231, 536 241, 536 245, C12Q 168, C12N 1510

Patent

active

055829724

ABSTRACT:
Methods are provided for preparing antisense oligonucleotides which take advantage of RNA secondary and tertiary structure and for preparing antisense oligonucleotides which specifically hybridize to regions of RNA secondary and tertiary structure by comparing the affinity of the oligonucleotide for a structured RNA target to the affinity of the oligonucleotide for a length-matched oligonucleotide complement, and selecting an oligonucleotide having an affinity for the structured target which is not less than one thousandth of its affinity for the length-matched oligonucleotide complement. Oligonucleotides are also disclosed which are specifically hybridizable with regions of H-ras RNA having secondary and tertiary structure.

REFERENCES:
patent: 5034506 (1991-07-01), Summerton
Ausubel et al., Current protocols in molecular biology 1989, John Wiley, New York.
Bacon, T. A. & Wickstrom, E., Walking Along Human c-myc mRNA with Antisense Oligonucleotides: Maximum Efficacy at the 5' Cap Region, Oncogene Res. 1991, 6, 13.
Bhattacharyya, A. et al., RNA Bulges and the Helical Periodicity of Double-Stranded RNA, Nature 1990, 343, 484.
Chow, S. A. et al., RecA Protein-promoted Homologous Pairing and Strand Exchange Between Intact and Partially Single-Stranded Duplex DNA, J. Mol. Biol. 223 1992, 79.
Chiang, M. Y. et al., Antisense Oligonucleotides Inhibit Intercellualr Adhesion Molecule 1 Expression by Two Distinct Mechanisms, J. Biol. Chem. 266 1991, 18162.
Fedor, M. J. & Uhlenbeck, O. C., Substrate Sequence Effects on "Hammerhead" RNA Catalytic Efficiency, Proc. Natl. Acad. Sci. USA 87 1990, 1668.
Freier, S. M. & Tinoco, I. Jr., The Binding of Complementary Oligoribonucleotides to Yeast Initiator Transfer RNA, Biochemistry 14 1975 3310.
Freier, S. M. et al., Contributions of Dangling End Stacking and Terminal Base Pair Formation to the Stabilities of XGGCCp, XCCGGp, XGGCCyp, and XCCGGYp Helixes, Biochemistry 1985, 24, 4533.
Fried, M. & Crothers, D. M. Equilibria and Kinetics of lac Repressor-Operator Interactions by Polyacrylamide Gel Electrophoresis, Nucl. Acids Res. 1981 9, 6505.
Garner, M. M. & Revzin, A., A Gel Electrophoresis for Quantifyng the Binding of Proteins to Specific DNA Regions: Application to Components of the Escherichia Coli Operon Regulatory System, Nucl. Acids Res. 1981, 9, 3047.
Gutell, R. R. et al., Comparative Anatomy of 16-S-lie Ribosomal RNA, Prog. Nucleic Acids Res. and Mol. Biol. 1985, 32, 155.
Herschlag, D. & Cech, T. R., Catalysis of RNA Cleavage by the Tetrahymena Thermophila Ribozyme, Biochemistry 29 1990a, 10159.
Herschlag, D. & Cech, T. R., Catalysis of RNA Cleavage by the Tetrahymena Thermophila Ribozyme, Biochemistry 29 1990b, 10172.
Jaeger, J. A. et al., Improved Predictions of Secondary Structures for RNA, Proc. Natl. Acad. Sci. USA 1989, 86, 7706.
Jinno, Y. et al., A Novel Effect of EGF on mRNA Stability, Nucl. Acids Res. 16 1988, 4957.
P. E. Nielsen, M. et al., Sequence-Seelctive Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide, Science 1991, 254, 1497.
Pyle, A. M. et al., Direct Measurement of Oligonucleotide Substrate Binding to Wild-Type and Mutant Ribozymes from Tetrahymena, Proc. Natl. Acad. Sci. USA 1990, 87, 8187.
Pontius, B. W. & Berg, P., Rapid Renaturation of Complementary DNA Strands Mediated by Cationic Detergents: A Role for High-Probability Binding Domains in Enhancing the Kinetics of Molecular Assembly Processes, Proc. Natl. Acad. Sci. USA 1991, 88, 8237.
Reddy, E. P., Nucleotide Sequence Analysis of the T24 Human Bladder Carcinoma Oncogene, Science 1983, 220, 1061.
Revzin, A., Gel Electrophoresis Assays for DNA-Protein Interactions, Biotechniques 1989, 7, 346.
Sambrook et al., Molecular Cloning. A Laboratory Manual, Second Edition 1989, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
Uhlenbeck, O. C., Complementary Oligonucleotide Binding to Transfer RNA, J. Mol. Biol. 65 1972, 25.
Wickstrom, E. et al., Complementary Oligonucleotide Probe of Vesicular Stomatitis Virus Matrix Protein mRNA Translation, Biophys. J. 49 1986, 15.
Wu, T. & Ogilvie, K. K., A Study on the Alkylsilyl Groups in Oligoribonucleotide Synthesis, J. Org. Chem. 55 1990, 4717.
Wu, T. et al., Preventiton of Chain Cleavage in the Chemical Synthesis of 2'-silylated oligoribonucleotides, Nucleic Acids Res. 17 1989, 3501.
Yoon, K. et al., The Kinetics of Codon-Anticodon Interaction in Yeast Phenylalanine Transfer RNA, J. Mol. Biol. 1975, 99, 507.
Fedor et al. (1990) PNAS, USA 87, 1668-1672.
Uhlmann et al. (1990) Chemical Review vol. 90(4):544-579.
Lima et al. (1992) Biochemistry vol. 31:12055-12061.
Monia et al. (1992) J. Biol. Chem. vol. 267(28):19954-19962.
Daoka et al. (1990) Oncogene Research vol. 5: 267-275.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Antisense oligonucleotides to the RAS gene does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antisense oligonucleotides to the RAS gene, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antisense oligonucleotides to the RAS gene will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-423148

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.