Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2004-09-13
2009-06-16
McGarry, Sean (Department: 1635)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S325000, C435S375000, C536S023100, C536S024300, C536S024330, C536S024500
Reexamination Certificate
active
07547511
ABSTRACT:
Using gene array technology, we observed that an increase of the α4 chain-containing Laminin-8 correlated with poor prognosis for patients with brain gliomas. We established that inhibition of Laminin-8 expression by a new generation of highly specific and stable antisense oligonucleotides (Morpholino™) against chains of Laminin-8 could slow or stop the spread of glioma. This was demonstrated in an in vitro model using human glioblastoma multiforme cell lines M059K and U-87MG co-cultured with normal human brain microvascular endothelial cells (HBMVEC). Using Western blot analysis and immunohistochemistry, we con-firmed that antisense treatment effectively blocked laminin-8 protein synthesis. Antisense oligonucleotides against both α4 and β1 chains of laminin-8 blocked significantly the invasion of co-cultures through Matrigel. The results show that Laminin-8 may not only contribute to glioma progression and recurrence as part of the neovascularization process but also by directly increasing the invasive potential of tumor cells.
REFERENCES:
patent: 2005/055980 (2005-06-01), None
Khazenzon et al. Antisense inhibition of laminin-8 expression reduces invasion of human gliomas in vitro. Molecular Cancer Therapeutics, 2003 vol. 2:985-994.
Fujita et al. Inhibition of laminin-8 in vivo using a novel poly(malic acid)-based carrier reduces glioma angiogenesis. Angiogenesis, 2006 vol. 9:183-191.
Lu et al. (2005). Deliverying siRNA in vivo for functional genomics and novel therapeutics. From RNA Interference Technology (Cambridge, Appasani, ed., pp. 303-317).
Samarsky et al. (2005). RNAi in drug development: Practical considerations. From RNA Interference Technology (Cambridge, Appasani, ed., pp. 384-395).
Nielsen, PE. Systemic delivery. The last hurdle? Gene Therapy, 2005 vol. 12:956-957.
Agrawal et al. Antisense therapeutics: is it as simple as complementary base recognition? Molecule Medicine Today, Feb. 2000 vol. 6:72-81.
Gewirtz et al. Facilitating oligonucleotide delivery: helping antisense delivery on its promise. Proc. Natl. Acad. Sci., 1996 vol. 93:3161-3163.
Ljubimova et al., Gene Array Analysis Of Differentially Expressed Genes In Human Glial Tumors. International Journal of Oncology, (2001), pp. 287-295, 18.
Ljubimova et al., Overexpression Of a4 Chain-Containing Laminins In Human Glial Tumors Identified By Gene Microarray Analysis, Cancer Research (Jul. 15, 2001), pp. 5601-5610, 61(14).
Sehgal, A., Molecular Changes During The Genesis Of Human Gliomas, Seminars In Surgical Oncology, (Jan.-Feb. 1998), pp. 3-12, 14(1).
Lal et al., A Public Database For Gene Expression In Human Cancers. Cancer Research, (Nov. 1, 1999), pp. 5403-5407, 59(21).
Miner et al., The Laminin Alpha Chains: Expression, Developmental Transitions, and Chromosomal Locations Of A1-5, Identification Of Heterotrimeric Laminins 8-11, and Cloning Of A Novel a3 Isoform, Journal of Cell Biology, (May 5, 1997), pp. 685-701, 137(3).
Colognato et al., Form And Function: The Laminin Family Of Heterotrimers, Developmental Dynamics, (Jun. 2000), pp. 213-234, 218(2).
Patarroyo et al., Laminin Isoforms In Tumor Invasion, Angiogenesis And Metastasis, Seminars . Seminars In Cancer Biology, (Jun. 2002), pp. 197-207, 12(3).
Belkin et al., Integrins As Receptors For Laminins, Microscopy Research and Technique, (Nov. 1, 2000), pp. 280-301, 51(3).
Kulla et al., Tenascin Expression Patterns and Cells Of Monocyte Lineage: Relationship In Human Gliomas, Modern Pathology, (Jan. 2000), pp. 56-67, 13(1).
Sixt et al., Endothelial Cell Laminin Isoforms, Laminins 8 And 10, Play Decisive Roles In T Cell Recruitment Across The Blood-Brain Barrier In Experimental Autoimmune Encephalomyelitis Journal of Cell Biology, (May 28, 2001), pp. 933-946, 153(5).
Thyboll et al., Deletion Of The Laminin a4 Chain Leads To Impaired Microvessel Maturation, Molecular and Cellular Biology, (Feb. 2002), pp. 1194-1202, 22(4).
Fujiwara et al., Purification And Characterization Of Human Laminin-8. Laminin-8 Stimulates Cell Adhesion And Migration Through a3β1 and a6p, Integrins, Journal of Biological Chemistry, (May 18, 2001), pp. 17550-17558, 276(20).
Gonzalez et al., Complex Interactions Between The Laminin a4 Subunit And Integrins Regulate Endothelial Cell Behavior In Vitro And Angiogenesis In Vivo, Proceedings of the National Academy of Sciences USA, (Dec. 10, 2002), pp. 16075-16080, 99(25).
Astriab-Fisher et al., Antisense Inhibition Of P- Glycoprotein Expression Using Peptide-Oligonucleotide Conjugates, Biochemical Pharmacology (Jul. 1, 2000), pp. 83-90, 60(1).
McKean et al., FAK induces Expression Of Prx1 To Promote Tenascin-C-Dependent Fibroblast Migration, Journal Of Cell Biology, (Apr. 28, 2003), pp. 393-402, 161(2).
Petajaniemi et al., Localization Of Laminin a4-Chain In Developing And Adult Human Tissues, The Journal Of Histochemistry and Cytochemistry, (Aug. 2002), pp. 1113-1130, 50(8).
Ljubimov et al., Human Corneal Basement Membrane Heterogeneity: Topographical Differences In The Expression Of Type IV Collagen And Laminin Isoforms, Lab Investigation, (Apr. 1995), pp. 461-473, 72(4).
Albini et al., A Rapid In Vitro Assay For Quantitating The Invasive Potential Of Tumor Cells, Cancer Research, (Jun. 15, 1987), pp. 3239-3245, 47(12).
Kleinman et al., Basement Membrane Complexes With Biological Activity, Biochemistry, (Jan. 28, 1986), pp. 312-318, 25(2).
Minakawa et al., In Vitro Interaction Of Astrocytes And Pericytes With Capillary-Like Structures Of Brain Microvessel Endothelium, Lab Investigation, (Jul. 1991), pp. 32-40, 65(1).
Voyta et al., Identification And Isolation Of Endothelial Cells Based On Their Increased Uptake Of Acetylated-Low Density Lipoprotein, Journal Of Cell Biology, (Dec. 1984), pp. 2034-2040, 99(6).
Herold-Mende et al., Clinical Impact And Functional Aspects Of Tenascin-C Expression During Glioma Progression, International Journal Of Cancer, (Mar. 20, 2002), pp. 362-369, 98(3).
Zagzag et al., Angiogenesis In The Central Nervous System: A Role For Vascular Endothelial Growth Factor/Vascular Permeability Factor and Tenascin-C. Common Molecular Effectors In Cerebral Neoplastic And Non-Neoplastic “Angiogenic Diseases”, Histol Histopathol, 17: 301-321, 2002.
Qin et al., The Transcription Factors Sp1, Sp3, and AP-2 Are Required For Constitutive Matrix Metalloproteinase-2 Gene Expression In Astroglioma Cells, Journal Of Biological Chemistry, (Oct. 8, 1999), pp. 29130-29137, 274(41).
Kachra et al., Expression Of Matrix Metalloproteinases And Their Inhibitors In Human Brain Tumors, Clinical and Experimental Metastasis, (1999), pp. 555-566, 17(7).
MacDonald et al., Urokinase Induces Receptor Mediated Brain Tumor Cell Migration And Invasion, Journal Of Neuro-Oncology, (Dec. 1998), pp. 215-226, 40(3).
Tsuj et al., Regulation Of Melanoma Cell Migration And Invasion By Laminin-5 And a3β1 Integrin (VLA-3), Clinical and Experimental Metastasis, (2002), pp. 127-134, 19(2).
Kondraganti et al., Selective Suppression Of Matrix Metalloproteinase-9 In Human Glioblastoma Cells By Antisense Gene Transfer Impairs Glioblastoma Cell Invasion, Cancer Research, (Dec. 15, 2000), pp. 6851-6855, 60(24).
Nielsen et al., Peptide Nucleic Acid Targeting Of Double-Stranded DNA. Methods In Enzymology, 2001, pp. 329-340, 340.
Dias et al., Antisense Oligonucleotides : Basic Concepts and Mechanisms, Molecular Cancer Therapy, (Mar. 2002), pp. 347-355, 1(5).
Summerton et al., Morpholino Antisense Oligomers: Design, Preparation And Properties, Antisense And Nucleic Acid Drug Development, (Jun. 1997) pp. 187-195, 7(3).
Lacerra et al., Restoration Of Hemoglobin A Synthesis In Erythroid Cells From Peripheral Blood Of Thalassemic Patients, Proceedings of the National Academy Of Sciences USA, (Aug. 15, 2000), pp. 9591-9596, 97(17).
Taylor et al., Comparison Of Efficacy Of Antisense Oligomers Directed Toward TNF-a In Helper T And Macrophage Cell Lines, Cytokine, (Sep. 1997), pp. 672-681, 9(9).
Arora et al., c- Myc Antisense Limits Rat Liver Regeneration And Indicates Role For c-myc In Regulating Cytochrome P-450 3A Activity,
Black Keith L.
Ljubimov Alexander V.
Ljubimova Julia Y.
Cedars-Sinai Medical Center
Davis , Wright, Tremaine, LLP
Gibbs Terra Cotta
Levy Seth D.
McGarry Sean
LandOfFree
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