Antisense inhibition of hepatitis B virus replication

Details Antisense inhibition of hepatitis B virus replication Antisense inhibition of hepatitis B virus replication

1995-07-13

1999-11-16

Degen, Nancy

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of regulating cell metabolism or physiology

536 245, C12N 508, C12N 706, C07H 2100, C07H 2104

Patent

active

059856629

ABSTRACT:
Antisense oligonucleotides are provided which are capable of inhibiting HBV replication. These oligonucleotides are specifically hybridizable with HBV RNAs which encode a P gene product, S gene product or C gene product, or with the 5' cap region, U5 region, .epsilon. region or translation initiation site of HBV RNA. Methods of diagnosing HBV infection, methods of inhibiting HBV replication, methods of treating an HBV infection and methods of treating or preventing HBV-associated diseases using the oligonucleotides of the invention are also provided. Such diseases may include acute hepatitis, chronic hepatitis, fulminant hepatitis, or hepatocellular carcinoma.

REFERENCES:
patent: 4806463 (1989-02-01), Goodchild et al.
patent: 5004810 (1991-04-01), Draper
patent: 5166195 (1992-11-01), Ecker
patent: 5194428 (1993-03-01), Agrawal et al.
patent: 5264423 (1993-11-01), Cohen et al.
patent: 5276019 (1994-01-01), Cohen et al.
patent: 5646262 (1997-07-01), Korba et al.
patent: 5728518 (1998-03-01), Carmichael
Gura et al. "Antisense has Growing Pains" Science 270: 575-577, Oct. 1995.
Stull et al. "Antigene, Ribozyme, andd Aptamer Nucleic Acid Drugs: Prospect and Progress" Pharmaceutical Res. 12(4): 465-481, Apr. 1995.
Whitton "Antisense treatment of Viral Infection" Adv. Virus Res. 44: 267-303, 1994.
Zon et al. in "Oligonucleotides and Analogues: A Practical Approach" F. Eckstein, ed. IRL Press, New York, pp. 87-108, 1991.
Current Protocols in Molecular Biology, Frederick M. Ausubel et al., eds., John Wiley & Sons Inc., 1994, Section 2.9B.
Blum et al., "Inhibition of hepatitis B. virsu by antisense oligodeoxynucleotides", Lancet 1991, 337, 1230.
Cornelius, C.E., 1988, in The Liver: Biology and Pathobiology, Second Ed. I.M. Arias et al., eds. Raven Press, Ltd., N.Y., pp. 1315-1336.
Junker-Niepmann et al., "A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA", EMBO J. 1990, 10, 3389-3396.
Korba and Gerin, "Use of standardized cell culture assay to assess activities of nucleoside analogs against hepatitis B virus replication", Antiviral Res. 1992, 19, 55-70.
Korba and Milman, "A cell culture assay for compounds which inhibit hepatitis B virus replication", Antiviral Res. 1991, 15, 217-228.
Gerin, J.L. 1984. In Advances in Hepatitis Research, F. Chisari, ed. Masson Publishing USA, Inc. New York, pp. 40-48.
Gerin et al. 1986 In Vaccines 86: New approaches to Immunization., F. Brown et al., eds. Cold Spring Harbor Laboratory Press, N.Y., pp. 383-386.
Goodarzi et al., "Antisense oligodeoxyribonucleotides inhibit the expression of the gene for hepatitis B virus surface antigen", J. Gen. Virol. 1990, 71, 3021-3025.
Miller and Robinson, "Common evolutionary origin of hepatitis B virus and retroviruses", Proc. Natl. Acad. Sci. 1986, 83, 2531-2535.
Offensperger et al., "In vivo inhibition of duck hepatitis B virus replication and gene expression by phosphorothioate modified antisense oligodeoxynucleotides", EMBO J. 1993, 12, 1257-1262.
Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY, pp. 7.53-7.55 and pp. 9.31-9.58.
Maister, Philippa,"Isis' Antisense Drug Shows Promise for Retinitis", BioWorld Today, Apr. 29, 1994, p. 3.
Takahashi et al., "Acute hepatitis in rats expressing human hepatitis B virus transgenes", Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 1470-1474.
Tennant, B.C. and J.L. Gerin. 1994. In The Liver: Biology and Pathobiology, Third Edition. I.M. Arias et al., eds. Raven Press, Ltd., N.Y. pp. 1455-1466.
Yao et al., Chung Hua I Hsueh Tsa Chih 1994, 74, 74-76.
Wu and Wu, "Specific Inhibition of Hepatitis B Viral Gene Expression in Vitro by Targeted Antisense Oligonucleotides", J. Biol. Chem. 1992, 267, 12436-12439.

Affiliated with

Also associated with

Rating

Antisense inhibition of hepatitis B virus replication does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antisense inhibition of hepatitis B virus replication, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antisense inhibition of hepatitis B virus replication will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1323697