Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-10-16
1999-01-12
Elliott, George C.
Chemistry: molecular biology and microbiology
Vector, per se
435 911, 536 231, 536 245, C12P 1934, C12N 1500, C07H 2102, C07H 2104
Patent
active
058587748
ABSTRACT:
A gene is regulated by introducing into a cell an inducible, tissue-specific antisense DNA construct. The antisense DNA construct comprises any inducible, tissue-specific gene, into which a DNA sequence antisense to any DNA sequence of the gene targeted for regulation has been inserted. The inducible, tissue-specific antisense DNA construct transcribes a hybrid messenger RNA containing an RNA sequence antisense to a sequence of the messenger RNA of the gene targeted for regulation. The hybrid messenger RNA also contains the RNA sequence of the inducible, tissue-specific gene. Some examples of suitable inducible genes include those selected from the group consisting of mammalian cytosolic phosphoenolpyruvate carboxykinase (PEPCK) (GTP, EC 4.1.1.32), mammalian atrial natriuretic factor (ANF), and mammalian alpha myosin heavy chain (.alpha.-MHC). In a preferred embodiment, the inducible, tissue-specific gene is the rat PEPCK gene.
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Malbon Craig C.
Moxham Christopher M.
Elliott George C.
The Research Foundation of State University of New York
Wang Andrew
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