Antiproliferative SgK reagents and methods

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007230

Reexamination Certificate

active

07087392

ABSTRACT:
The invention provides methods and compositions relating to activated Sgk and the association of activated Sgk activity with proliferating cells, including methods for inhibiting the growth of an undesirably proliferating cell by contacting the cell with a specific inhibitor of activated Sgk whereby the amount of activated Sgk activity in the cell is reduced. Specific inhibitors are disclosed, including dominant negative mutants of activated Sgk, an Sgk mutant or fragment thereof comprising an unphosphorylatable residue corresponding to Thr256 of native Sgk, and an active Sgk-specific antibody or antibody fragment, especially intrabody, etc. A wide variety of methods may be used to contact the target cell with the inhibitor, especially introducing into the cell a polynucleotide encoding the inhibitor under conditions whereby the inhibitor is expressed in the cell.

REFERENCES:
patent: 2001/0027184 (2001-10-01), Kumar et al.
patent: 0 887 081 (1998-12-01), None
Webster et al. Mol. Cell. Biol., 1993, 13(4): 2031-2040.
Delmolino et al., 1997, Journal of Cellular Physiology 173:371-79.
Park et al., 1999, The EMBO Journal 18(11):3024-33.

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