Antimicrobial proteins, compositions containing same and uses th

Details Antimicrobial proteins, compositions containing same and uses th Antimicrobial proteins, compositions containing same and uses th

1994-03-08

1997-08-05

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

536 231, 536 235, 4351723, 4353201, 4352523, 435372, 935 9, 935 24, 935 70, C12P 2102, C12P 1934, C12N 1506, C12N 1512

Patent

active

056541678

ABSTRACT:
The present invention provides purified polypeptides useful as antimicrobial agents. These polypeptides comprise human polymorphonuclear leukocyte polypeptides having molecular weights of about 25,000 daltons, 29,000 daltons and 54,000 daltons. These polypeptides have respiratory burst-independent, antibacterial activity at a pH from about 5.0 to about 8.0, at calcium ion concentrations up to about 10 mM, and at sodium chloride concentrations up to about 0.3M.

REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4468464 (1984-08-01), Cohen et al.
patent: 4675285 (1987-06-01), Clark et al.
patent: 4727028 (1988-02-01), Santerre et al.
patent: 5091303 (1992-02-01), Amaout et al.
Morgan, J.G., (1991) "Cloning the cDNA for the Serine Protease Homolog CAP 37/Azurocidin, A Microbicidal and Chemotactic Protein from Human Granulocytes," The J. of Immunology 147: 3210-3214 (Exhibit 1).
Campanelli, D. et al., (1990) "Azurocidin and a Homologous Serine Protease from Neutrophils, Differential Antimicrobial and Proteolytic Properties" J. Clin. Invest. 85: 904-915 (Exhibit 2).
Pereira, H. A. et al., (1990) "CAP37, a Human Neutrophil-Derived Chemotactic Factor with Monocyte Specific Activity" J. Clin. Invest. 85 (5) 1468-1476 (Exhibit 3) --Abstract only.
Pereira, H.A. (1990) "CAP 37, a 37 kD Human Neutrophil Granule Cationic Protein Shares Homology with Inflammatory Proteinases" 46 (3): 189-196 (Exhibit 4) Abstract Only.
Hanson, R.D. et al., (1990) "A Cluster of Hematopoietic Serine Protease Genes Is Found on the Same Chromosomal Band as the Human Alpha/Delta T-Cell Receptor Locus" Proc. Natl. Acad. Sci. 87 (3): 960-963 U.S.A. (Exhibit 5) Abstract only.
Campanelli, D. et al., (1990) "Cloning of cDNA for Proteinase 3: A Serine Protease, Antibiotic, and Autoantigen from Human Neutrophils" J. Exp. Med. 172:1709-1715 (Exhibit 6).
Rao, N.V. et al., (1991) "Characterization of Proteinase-3 (PR-3), a Neutrophil Serine Proteinase" J. of Biol. Chem. 266 (15):9540-9548 (Exhibit 7).
Bories, D. et al., (1989) "Down-Regulation of a Serine Protease, Myeloblastin, Causes Growth Arrest and Differentiation of Promyelocytic Leukemia Cells" 59:959-968 (Exhibit 8).
Sturrock, A.B. et al., (1992) "Structure, Chromosomal Assignment, and Expression of the Gene for Proteinase-3, the Wegener's Granulomatosis Autoantigen" J. of Biol. Chem. 267 (29):21193-21199 (Exhibit 9).
Kao, R.C. et al., (1988) "Proteinase 3 A Distinct Human Polymorphonuclear Leukocyte Proteinase that Produces Emphysema in Hamsters" J. Clin. Invest. 82:1963-1973 (Exhibit 10).
Kam, C-M et al., (1992) "Substrate and Inhibitor Studies on Proteinase 3" FEBS Letters 297 (1,2):119-123 (Exhibit 11).
McGrogan, M. et al., (1988) "Isolation of a Complementary DNA Clone Encoding a Precursor to Human Eosinophil Major Basic Protein" J. Exp. Med. 168:2295-2308 (Exhibit 12).
Hastie, A. T. et al. (1987) "The Effect of Purified Human Eosinophil Major Basic Protein on Mammalian Ciliary Activity" Am. Rev. Respir. Dis. 135:848-853 (Exhibit 13).
Wasmoen, T. L. et al., (1988) "Biochemical and Amino Acid Sequence Analysis of Human Eosinophil Granule Major Basic Protein" J. of Biol. Chem. 263 (25): 12559-12563 (Exhibit 14).
Gleich, G. J. et al., (1979) "Cytotoxic Properties of the Eosinophil Major Basic Protein" J. of Immun. 123 (6):2925-2927 (Exhibit 15).
Weller, P. F. et al. (1988) "Eosinophil Granule Cationic Proteins: Major Basic Protein is Distinct from the Smaller Subunit of Eosinophil Peroxidase" J. of Leukocyte Biol. 43:1-4 (Exhibit 16).
Butterworth, A. E. et al. (1979) "Damage to Schistosomula of Schistosoma Mansoni Induced Directly by Eosinophil Major Basic Protein" J. of Immun. 122 (1):221-229 (Exhibit 17).
Frigas, E. et al. (1986) "The Eosinophil and the Pathophysiology of Asthma" J. Allergy Clin. Immunol. 77 (4):527-537 (Exhibit 18).
Weiler, D. et al. (1989) "Mast Cells in Carnoy's Fixed Tissue Stain Positively for Eosinophil Major Basic Protein (MBP)" Federation Proceedings Abstract 3612 (Exhibit 19).
Wasmoen, T.L. et al. (1987) "Biochemical and Amino Acid Sequence Analysis of Eosinophil Granule Major Basic Protein" Federation Proceedings Abstract 3577 (Exhibit 20).
Slifman, N. et al. (1987) "Eosinophil-Derived Neurotoxin and Eosinophil Protein-X: Comparison of Physicochemical, Immunologic and Enzymatic Properties" Federation Proceedings Abstract 3578 (Exhibit 21).
O'Donnell, M. C. et al. (1983) "Activation of Basophil and Mast Cell Histamine Release by Eosinophil Granule Major Basic Protein" J. Exp. Med. 157:1981-1991 (Exhibit 22).
Wasmoen, T. L. et al. (1987) "Increases of Plasma Eosinophil Major Basic Protein Levels Late in Pregnancy Predict Onset of Labor" Proc. Natl. Acad. Sci. USA 84:3029-3032 (Exhibit 23).
Peters, M. S. et al. (1987) "Extracellular Deposition of Eosinophil Granule Major Protein In Pressure Urticaria" J. Am. Acad. Dermatol. 16 (3) (I):513-517 (Exhibit 24).
Jenne et al., 1990, Nature 346, p. 520.
Froohney, I.R. 1983. in: Culture of Animal Cells A Manual of Basic Technique. Alan R. Lins, Inc. NY. p. 167.
Young et al. 1983 Proc. Natl. Acad Sci. USA 80, 1194-1198.
Suggs et al. 1981. Proc. Natl. Acad. Sci. USA. 78, 6613-6617.
Thilly (ed.) 1986. in: Mammalian Cell Technology. Butterworths., Boston pp. 63-89.
Maddox et al. 1984. J. Exp. Med. 160, 29-41.
Fischkoff et al. 1984. J. Exp. Med. 160, 179-196.
Gabay et al. 1989. Proc. Natl. Acad. Sci. USA 86, 5610-5614.
Heck et al. 1986. Anal. Biochem. 158, 217-227.
Sequence Comparison (Alignment) of sequence ident. No. 23, with that of Barker et al. (J. Exp. Med. vol. 68, of record).
Ohlsson et al. 1990. Biol. Chem. Hoppe-Seyler. 371, 549-555.
Klein, J. 1982 in: Immunology. The Science of Self-Nonself Discrimination. John Wiley and Sons. New York p. 101.
Okayama et al. (1983) Molec. Cell. Biol. 3, 280-289.
Heron, E. J. (1984) Am. Biotechnol Lab. 2, 52-54, 56, 58-59.
Hunkapiller et al. (1983) Meth. Enzymol. 91, 399-413.
Wallace et al. (1981) Nuc. Acids Res. 9, 879-894.
Barker et al. J. Exp. Med. 168, 1493-1498.
Collins et al. (1977) Nature 270, 347-349.
Rest et al. (1978) Infec. Immun. 19, 131-137.
Maniatis et al. (eds.) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. pp. 188-199.

Affiliated with

Also associated with

Rating

Antimicrobial proteins, compositions containing same and uses th does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Antimicrobial proteins, compositions containing same and uses th, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Antimicrobial proteins, compositions containing same and uses th will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1074159