Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase
Reexamination Certificate
2007-06-19
2007-06-19
Kerr, Kathleen M. (Department: 1656)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Oxidoreductase
C435S183000
Reexamination Certificate
active
11033116
ABSTRACT:
The object of the present invention is to screen and identify a novel antimicrobial protein which can inhibit the growth of plant pathogenic microorganisms at a relatively low concentration such asPyricularia oryzaeandRhizoctonia solanicausative of two major diseases causing damage to rice crops and, further, to clone the gene of this protein. According to the present invention, an antimicrobial protein which can be obtained from a fraction of an aqueous extract ofLyophyllum shimejiprecipitated by the ammonium sulfate precipitation method, has an antimicrobial activity at least againstRhizoctonia solaniorPyricularia oryzae, and shows the presence of components of about 70 kDa and/or about 65 kDa in molecular weight in the SDS-PAGE method. A gene encoding this protein and a method of using the same are provided.
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Inoue Yasuhiro
Kuwata Shigeru
Takakura Yoshimitsu
Corporate Juridical Person Society for Techno-Innovation of Agri
Japan Tobacco Inc.
Kerr Kathleen M.
Noakes Suzanne M.
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