Antimicrobial peptides from bovine neutrophils

Details Antimicrobial peptides from bovine neutrophils Antimicrobial peptides from bovine neutrophils

1994-12-13

1998-10-13

Wax, Robert A.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

530324, 530300, 5303879, 5303881, 5303891, 514 2, 435 691, 536 235, A61K 3817, C07K 1447, C07K 1618, C12N 1512

Patent

active

058212248

ABSTRACT:
The present invention provides a new family of cysteine-rich antimicrobial peptides isolated from bovine neutrophils herein named beta defensins. Thirteen structurally homologous peptides were purified to homogeneity from a granule-rich cytoplasmic fraction of purified blood neutrophils. These antimicrobial compounds are useful in human and veterinary medicine, and as agents in agricultural, food science, and industrial applications.

REFERENCES:
patent: 5202420 (1993-04-01), Zasloff et al.
patent: 5459235 (1995-10-01), Selsted et al.
patent: 5550109 (1996-08-01), Schonwetter et al.
patent: 5635594 (1997-06-01), Lehrer et al.
Lehrer et al., "Defensins: Endogenous Antibiotic Peptides of Animal Cells" Cell 64:229-230 (1991).
Selsted et al., "Determination of the Disulfide Array in the Human Defensin HNP-2" J. Biol. Chem. 264(7):4003-4007 (1989).
Bidlingmeyer et al., "Rapid Analysis of Amino Acids Using Pre-Column Derivatization" J. Chromatogr. 336:93-104 (1984).
Klemm, P., "Manual Edman Degradation of Proteins and Peptides" In: Methods in Molecular Biology. Walker, J.M. ed. Human Press, Clifton, NJ 1:243-254 (1984).
Edelhoch, H., "Spectroscopic Determination of Tryptophan and Tyrosine in Proteins" Biochemistry 6(7):1948-1954 (1967).
Pardi et al., "Solution Structures of the Rabbit Neutrophil Defensin NP-5" J. Mol. Biol. 201:625-636 (1988).
Hill et al., "Crystal Structure of Defensin HNP-3, an Amphiphilic Dimer: Mechanisms of Membrane Permeabilization" Science 251:1481-1485 (1991).
Lehrer et al., "Antimicrobial Polypeptides of Human Neutrophils" Blood 76(11):2169-2181 (1990).
Spitznagel, J.K., "Antibiotic Proteins of Human Neutrophils" J. Clin. Invest. 86:1381-1386 (1990).
Gennaro et al., "Neutrophil and Eosinophil Granules as Stores of Defense Proteins" In: Blood Cell Biochemistry, J.F. Harris, ed. Plenum Press, New York 335-368 (1991).
Ganz et al., "Defensins" Eur. J. Haematol 44:1-8 (1990).
Ganz et al., "Natural Peptide Antibiotics of Human Neutrophils" J. Clin. Invest. 76:1427-1435 (1985).
Selsted et al., "Primary Structures of Six Antimicrobial Peptides of Rabbit Peritoneal Neutrophils" J. Biol. Chem. 260(8):4579-4584 (1985).
Eisenhauer et al., "Purification and Antimicrobial Properties of Three Defensins from Rat Neutrophils" Infect. Immun. 57(7):2021-2027 (1989).
Eisenhauer et al., "Polymorphic Expression of Defensins in Neutrophils from Outbred Rats" Infect. Immun. 58(12):3899-3902 (1990).
Selsted et al., "Purification, Primary Structure, and Antimicrobial Activities of a Guinea Pig Neutrophil Defensin" Infect. Immun. 55(9):2281-2286 (1987).
Yamashita et al., "Purification, Primary Structure, and Biological Activity of Guinea Pig Neutrophil Cationic Peptides" Infect. Immun. 57:2405-2409 (1989).
Gennaro et al., "Potency of Bactericidal Proteins Purified from the Large Granules of Bovine Neutrophils" Infect. Immun. 40:684-690 (1983).
Gennaro et al., "A Novel Type of Cytoplasmic Granule in Bovine Neutrophils" J. Cell. Biol. 96:1651-1661 (1983).
Baggiolini et al., "Identification of Three Types of Granules in Neutrophils of Ruminants" Lab. Invest. 52(2):151-158 (1985).
Romeo et al., "Structure and Bactericidal Activity of an Antibiotic Dodecapetide Purified from Bovine Neutrophils" J. Biol. Chem. 263(20):9573-9575 (1988).
Frank et al., "Amino Acid Sequence of Two Proline-rich Bactenecins" J. Biol. Chem. 265(31):18871-18874 (1990).
Gennaro et al., "Purification, Composition, and Activity of Two Bactenecins, Antibacterial Peptides of Bovine Neutrophils" Infect. Immun. 57(10):3142-3146 (1989).
Selsted et al., "Indolicidin: a Novel Bactericidial Tridecapeptide Amide from Neutrophils" J. Biol. Chem. 267(7):4292-4295 (1992).
Amrein et al., "Prevention of Degradation of Human Polymorphonuclear Leukocyte Proteins by Diisopropylfluorophosphate" Blood 56(3):442-447 (1980).
Borregard et al., "Subcellular Localization of the b-Cytochrome Component of the Human Neutrophil Microbicidal Oxidase: Translocation during Activation" J. Cell. Biol. 97:52-61 (1983).
Fling et al., "Peptide and Protein Molecular Weight Determination by Electrophoresis Using a High-Molarity Tris Buffer System Without Urea" Anal. Biochem. 155:83-88 (1986).
Selsted et al., "Eosin Y: A Reversible Stain for Detecting Electrophoretically Resolved Protein" Anal. Biochem. 155:270-274 (1986).
Selsted et al., "Purification and Antibacterial Activity of Antimicrobial Peptides of Rabbit Granulocytes" Infect. Immun. 45(1):150-154 (1984).
Henschen, A.H., "5.1 Analysis of Cyst(e)ine Residues, Disulfide Bridges, and Sulfhydryl Groups in Proteins" In: Advanced Methods in Protein Microsequence Analysis, B. Wittmann-Liebold, J. Salnikow, and V.A. Erdman, eds. Springer-Verlag, Berlin 244-255 (1986).
Ambler, R.P., "Enzymatic Hydrolysis with Carboxypeptidases" Methods Enzymol 25:143-154 (1972).
Lehrer et al., "Ultrasensitive assays for endogenous antimicrobial polypeptides" J. Immunol. Methods 137:167-173 (1991).
Altschul et al., "Basic Local Alignment Search Tool" J. Mol. Biol.215:403-410 (1990).
Fischer et al., "Identification of a mammalian glutaminyl cyclase converting glutaminyl into pyroglutamyl peptides" Proc. Natl. Acad. Sci. USA 84:3628-3632 (1987).
Zhang et al., "NMR Studies of Defensin Antimicrobial Peptides. 1. Resonance Assignment and Secondary Structure Determination of Rabbit NP-2 and Human HNP-1" Biochem. 31(46):11348-11356 (1992).
Pardi et al., "NMR Studies of Defensin Antimicrobial Peptides. 2. Three-Dimensional Structures of Rabbit NP-2 and Human HNP-1" Biochem. 31(46):11357-11364 (1992).
Chang et al., "High-level secretion of human growth hormone by Escherichia coli" Gene 55:189-196 (1987).
Neumann et al., "Gene Transfer into Mouse Lyoma Cells By Electroporation in High Electric Fields" EMBO J. 1(7):841-845 (1982).
Wade et al., "All-D amino acid-containing channel-forming antibiotic peptides" Proc. Natl. Acad. Sci. USA 87:4761-4765 (1990).
Sevier et al., "Monoclonal Antibodies in Clinical Immunology" Clin. Chem. 27(11):1797-1806 (1981).
Diamond et al. (1991) Proc. Nat. Acad. Sci., USA 88: 3952-3956.
Ganz et al. (1994) Curr. Opinions Immunol. 6: 584-589.
Martin et al. (1995) J. Leucocyte Biol. 58: 128-136.

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