Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – 15 to 23 amino acid residues in defined sequence
Patent
1992-08-28
1995-06-13
Warden, Jill
Chemistry: natural resins or derivatives; peptides or proteins;
Peptides of 3 to 100 amino acid residues
15 to 23 amino acid residues in defined sequence
530300, A61K 3800, C07K 500, C07K 700, C07K 1700
Patent
active
054243955
ABSTRACT:
The present invention relates to a number of peptides which have antimicrobial activity and which are useful in retarding plant pathogens. In addition, compounds in accordance with the present invention may have improved resistance to degradation by plant proteases, and/or sufficiently low phytotoxicity to render them likely candidates for use in conjunction with plants. The present invention also relates to oligonucleotides which are capable of expressing the aforementioned peptides. Also provided hereby is a process of retarding the growth of plant pathogens using the compounds of the present invention as well as a screening method and screening reagent useful in determining the proteolytic degradation resistance of a known compound.
REFERENCES:
patent: 4458066 (1984-07-01), Caruthers et al.
patent: 4543252 (1985-09-01), Lehrer et al.
patent: 4810777 (1989-03-01), Zasloff
Dorper et al., "Improvements in the phosphoramidite procedure for the synthesis of oligodeoxyribonucleotides", Nucleic Acids Research, vol. 11, No. 9, 1983.
DeGreve et al., "Nucleotide Sequence and Transcript Map of the Agrobacterium tumefaciens Ti Plasmid-Encoded . . . ", Journal of Molecular and Applied Genetics, 499-511, 1983.
Winkler, "Biosynthesis of Histidine", Department of Molecular Biology, Northwestern University Medical School, 395-411.
Klement, "Method of Obtaining Fluid from the Intercellular Spaces . . . ", Phytopathology, vol. 55, 1033-1034.
Berkowitz et al., "Magainins: A New Family of Membrane-Active Host Defense Peptides", Biochemical Pharmacology, vol. 39, No. 4, pp. 625-629, 1990.
Zasloff et al., "Antimicrobial activity of synthetic magainin peptides and several analogues", Proc. Nat. Acad. Sci. USA, vol. 85, pp. 910-913, Feb. 1988.
Urrutia et al., "Spontaneous polymerization of the antibiotic peptide magainin 2", vol. 247, No. 1, 17-21.
Duclohier et al., "Antimicrobial peptide magainin 1 from Xenopus skin forms anion-permeable channels in planar lipid bilayers", Biophys. J., vol. 56, Nov. 1989, 1017-1021.
Rana et al., "Interactions between Salmonella typhimurium lipopolysaccharide and the antimicrobial peptide, magainin 2 amide", vol. 261, No. 2, 464-467.
Cuervo et al., "The Magainins: Sequence Factors Relevant to Increased Antimicrobial Activity and Decreased Hemolytic Activity", Peptide Research, vol. 1, No. 2 (1988), 81-86.
Zasloff, "Magainins, a class of antimicrobial peptides from Xenopus skin: Isolation, characterization of two active . . . ", Proc. Natl. Acad. Sci. USA, vol. 84, pp. 5449-5453, Aug. 1987.
Matsuzaki et al., "Magainin 1-induced leakage of entrapped calcein out of negatively-charged lipid vesicles", Biochimica et Biophysica Acta, 981 (1989) 130-134.
Rana et al., "Outer Membrane Structure in Smooth and Rough Strains of Salmonella typhimurium and Their . . . ", Biological and Synthetic Membranes, pp. 77-85, 1989.
Westerhoff et al., "Magainins and the disruption of membrane-linked free-energy transduction", Proc. Natl. Acad. Sci. USA, vol. 86, pp. 6597-6601, Sep. 1989.
Giovannini et al., "Biosynthesis and degradation of peptides derived from Xenopus laevis prohormones", Biochem. J. (1987) 243, 113-120.
Gordon-Kamm et al., "Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants", The Plant Cell, vol. 2, 603-618, Jul. 1990.
Tjoeng et al., "Multiple peptide synthesis using a single support (MPS3)", Int. J. Peptide Protein Res. 35, 1990, 141-146.
Horsch, "A Simple and General Method for Transferring Genes Into Plants", Science, vol. 227, pp. 1229-1231, Mar. 1985.
Hellebust et al., "Different Approaches to Stabilize a Recombinant Fusion Protein", Bio/Technology, vol. 7, pp. 165-168, Feb. 1989.
Zon et al., "A Review of High-Performance Liquid Chromatography in Nucleic Acids Research, II. Isolation, Purification, and . . . ", BioChromatography, vol. 1, No. 1, pp. 22-31, (1986).
Thompson, "A Review of High Performance Liquid Chromatography in Nucleic Acids Research, I. Historical Perspectives", BioChromatography, vol. 1, No. 1, pp. 16-20, (1986).
Gupta et al., "Development and Use of Chlorotetracycline Fluorescence as a Measurement Assay of Chloroplast Envelope-Bound Mg.sup.2+1 ", Platn Physiol., (1989) 89, 753-761.
Brosius, "Expression Vectors Employing .lambda.-, trp-, lac-, and 1pp-Derived Promoters", Chapter 10, pp. 205-225.
Caruthers, "New Methods for Chemically Synthesizing Deoxyoligonucleotides", Methods of DNA and RNA Sequencing, pp. 1-22.
Williams et al., "Raman Spectroscopy of Synthetic Antimicrobial Frog Peptides Magainin 2a and PGLa", Biochemistry 1990, 29, 4490-4496.
Chen et al., "Synthetic magainin analogues with improved antimicrobial activity", vol. 236, No. 2, 462-466, Aug. 1988.
Juretic et al., "Magainin 2 amide and analogues, Antimicrobial activity, membrane depolarization and susceptibility to proteolysis", vol. 249, No. 2, 219-223, Jun. 1989.
Chen et al., "Magainin analogs: A study of activity as a function of .alpha.-helix modification", Structural biology, pp. 122-123.
Cuervo et al., "Synthesis and antimicrobial activity of magainin alanine substitution analogs", Structural biology, pp. 124-126.
Cannon, "Antimicrobial peptides, A family of wound healers", Nature, vol. 328, Aug. 6, 1987, p. 478.
Nozaki, "Solid Phase Synthesis of Magainin 1 under Continuous Flow Conditions 1", Chemistry Letters, pp. 749-752, 1989.
Casteels et al., "Apidaecins: antibacterial peptides from honeybees", the EMBO Journal, vol. 8, No. 8, pp. 2387-2391, 1989.
Jaynes et al., "Increasing Bacterial Disease Resistance in Plants Utilizing Antibacterial Genes from Insects", BioEssays, vol. 6, No. 6, Jun. 1987, pp. 263-270.
Itakura et al., "Synthesis and Use of Synthetic Oligonucleotides", Ann. Rev. Biochem., 1984, 53:323-56.
Goldberg et al., "Cloning and expression of a collagen-analog-encoding synthetic gene in Escherichia coli", Gene, 80 (1989) 305-314.
Kuks et al., "Xenopus laevis Skin Arg-Xaa-Val-Arg-Gly-endoprotease", The Journal of Biological Chemistry, vol. 264, No. 25, Sep. 5, 1989, pp. 14609-14612.
Chen et al., "An extracellular matrix protein in plants: characterization of a genomic clone for carrot extensin", The EMBO Journal, vol. 4, No. 9, pp. 2145-2151, 1985.
Kini et al., "A common cytolytic region in myotoxins, hemolysins, cardiotoxins and antibacterial peptides", Int. J. Peptide Protein Res. 34, 1989, 277-286.
Bessalle et al.-Novel magainin analogues: Structure-activity relationship, Peptides 1990.
Maloy et al.-Design of broad spectrum antibiotic and host defense peptides based on magainin and related peptides, Peptides 1990.
Bascomb Newell F.
Everett Nicholas P.
Mapelli Claudio
Swerdloff Michael D.
Williams Jon I.
Davenport A. M.
Enichem S.p.A.
Warden Jill
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